高铜对肉鸡原代肝细胞周期及细胞膜电位的影响  

The effect of high copper on the cell cycle and cell membrane potential of primary hepatocyte in broilers

作　　者：郭剑英[1] 贾雪霞[1] 李静涛[1] 颜诚[1] 李海琴[1] 唐兆新[1,2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省兽用生物制品技术研究与应用企业重点实验室,广东肇庆510630

出　　处：《中国兽医学报》2013年第9期1448-1450,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30871900)

摘　　要：通过流式细胞仪测定各组的细胞周期及细胞膜电位,研究高铜对肉鸡原代肝细胞凋亡的影响。将全量换液24h后的细胞分为空白对照组和低铜组(10μmol/L Cu2+组)、高铜Ⅰ组(50μmol/L Cu2+组)、高铜Ⅱ组(100μmol/L Cu2+组),培养48h后测定荧光值,结果表明,高铜组能显著引起静止期(G0/G1)肝细胞数增多、增殖期(S、G2+M)肝细胞数减少、细胞膜电位下降(P<0.05);低铜组未出现明显变化;表明高浓度的铜能明显改变肝细胞的细胞周期及细胞膜电位。To investigate the effect of high copper on the cell cycle and cell membrance potential of the primary hepatocyte in broiler,the cell were divided into normal control,low copper group（10 μmol/L Cu2＋ group）,high copperⅠgroup（50 μmol/L Cu2＋group）and high copperⅡgroup（100 μmol/L Cu2＋ group）after changed the medium in 24h.The cell cycle and cell membrane potential were measured at 48hby the methods of flow cytometry（FCM）.The results showed that the G0 / G1 phase was significantly increased（P0.05）,and S phase,G2 ＋M phase were significantly decreased（P0.05）in high copper group.The relative fluorescence intesities（RFI）of cell membrane potential were decreased significantly in high copper group;There was no obvious change in low copper group.In conclusion,high copper could change the cell cycle and cell membrane potential of primary hepatocyte significantly.

关 键 词：铜离子 流式细胞仪 细胞周期 细胞膜电位 凋亡 

分 类 号：S852.2[农业科学—基础兽医学]