边鸡MSTN基因C.234位点对生长性状的遗传效应分析  

Genetic effect of the C.234 locus of the MSTN gene on growth traits in Bian chicken

作　　者：张跟喜[1] 曹宁贤 丁馥香[3] 王金玉[1] 戴国俊[1] 谢恺舟[1] 张丽[3] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]山西省畜禽繁育工作站,山西太原030032 [3]山西省农业科学院畜牧兽医研究所,山西太原030032

出　　处：《中国兽医学报》2013年第9期1470-1474,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31201793);江苏高校优势学科建设工程资助项目;扬州大学高层次人才科研启动基金;国家肉鸡产业技术体系资助项目(nycytx-42-G1-05);山西省科技攻关资助项目(20100311041)

摘　　要：采用PCR-RFLP技术检测连续2个世代边鸡群体肌肉生长抑制素基因外显子1的多态性,分析了多态位点对边鸡生长性状的遗传效应。结果表明,边鸡MSTN基因的C.234位点存在多态性,在外显子1编码区的234bp处发生G→A的碱基突变,产生了GG、GA和AA 3种基因型。关联分析表明,无论是一世代还是二世代的边鸡群体,在6~16周龄时,AA和GA基因型边鸡的体质量均显著或极显著的高于GG基因型（P〈0.05或P〈0.01）。结果提示,MSTN基因外显子1的C.234位点突变对边鸡的生长性状具有显著的遗传效应,初步推断该位点可用于边鸡生长性状的标记辅助选择。This study aimed to investigate the relationship between the MSTN gene and chicken growth traits,and search the marker correlated with chicken growth traits.Single nucleotide polymorphism（SNP）in exon 1 of the myostatin gene was detected by PCR-RFLP in Bian chicken of two consecutive generations.The results showed that C.234 locus displayed polymorphic.A G→ A mutation at the position of the 234 bp of coding region in exon 1 was found,which resulted in 3 genotypes（GG,GA,and AA）.Correlation analysis showed that,no matter for generation one or generation two,female Bian chickens of genotypes AA and GA had significantly higher body weight than those of genotype GG（P0.05or P0.01）from 6 to 16 weeks of age.These results suggest that the C.234 locus in exon 1 of the myostatin gene has significantly genetic effect on growth traits,and could be used as a genetic marker for growth traits in Bian chicken.

关 键 词：肌肉生长抑制素基因 边鸡 PCR-RFLP 生长性状 

分 类 号：S813.1[农业科学—畜牧学]