利奈唑胺对结核分枝杆菌体外抗菌活性的研究  

In vitro antibacterial activity of linezolid against Mycobacterium tuberculosis

作　　者：王平[1] 王晓燕[1] 刘国辉[1] 张瑛[1] 陈建波[1] 邓群益[1] 

机构地区：[1]深圳市第三人民医院肝病科,广东深圳518029

出　　处：《中华医院感染学杂志》2013年第17期4260-4261,共2页Chinese Journal of Nosocomiology

基　　金：深圳市结核病临床重点专科资助项目(2011-2015);深圳市结核病科技计划项目(201102066)

摘　　要：目的对比研究利奈唑胺对敏感及耐药结核分枝杆菌的体外抗菌活性，为今后的治疗提供依据。方法选取临床分离的敏感及耐药结核分枝杆菌各36株，分别在含有特定浓度的利奈唑胺等药物培养基内培育，采用B-D培养基比例法测定各菌株在体外试验的最低抑菌浓度（MIC）。结果利奈唑胺对敏感及耐药结核分枝杆菌的MIC均在0.250.50μg/ml，其中0. 125μg/ml浓度敏感株占63. 89% ,耐药株全部存活；在0.25μg/ml浓度下敏感株占33. 33%、耐药株占36. 11%，在0.5μg/ml浓度下敏感株占2. 77%、耐药株占63. 88%;〉1 Mg/ml仝部无细菌生长。结论常规剂k的利奈唑胺在体外有较好的抗结核分枝杆菌活性，虽然异烟肼、利福平对耐药结核分枝杆菌仍有较好的抗菌活性，但是需要增加剂量。OBJECTIVE To study the in vitro antibacterial activity of linezolid against sensitive and drug-resistantMycobacterium tuberculosis , so as to guide the treatment. METHODS Totally 36 clinical isolates of drug suscepti-ble M. tuberculosis and 36 clinical isolates of drug-resistant M. tuberculosis were chosen as the study objects andwere cultivated in media containing linezolid. The minimal inhibitory concentration （MIC） of in vitro test of various strains was determined by BD medium. RESULTS The MICs of linezolid on drug-sensitive and drug-resistantM. tuberculosis ranged between 0. 25 and 0. 50 μg/ml; of which the 0. 25 μg/ml drug-sensitive strains accountedfor 63. 89%,and all drug-resistant strains survived; under the concentration of 0. 25 μg/ml,the drug-sensitivestrains accounted for 33. 33%,and the drug-resistant strain accounted for 36. 11%; under the concentration of0. 5 μg/ml,the drug-sensitive strains accounted for 2. 77% , and the drug-resistant strains accounted for 63. 88% ;when the concentration was more than 1 μg/ml,no bacteria grew. CONCLUSION The linezolid routine dose of hasbetter in vitro antituberculotic activity. Although isoniazide and rifampicin have better antibacterial activity ondrug-resistant M. tuberculosis, it is necessary to add the dose.

关 键 词：利奈唑胺 体外抗菌活性 结核分枝杆菌 最低抑菌浓度 

分 类 号：R978.1[医药卫生—药品]