两株阿维菌素产量不同的菌株中相关基因的比较  

Comparative of Avermectin Related Genes in Two Strains with Deferent Avermectin Production

作　　者：杨晗[1,2] 钟娟[1,3] 孙丰慧[1,2] 舒丹[1,3] 罗笛[1,3] 谭红[1,3] 

机构地区：[1]中国科学院环境与应用微生物重点实验室,成都610041 [2]中国科学院大学,北京100049 [3]四川省环境微生物重点实验室,成都610041

出　　处：《生物技术通报》2013年第9期158-164,共7页Biotechnology Bulletin

基　　金：中国科学院环境与应用微生物重点实验室开放基金项目(KLCAS-2011-07)

摘　　要：以阿维菌素(Avermectin)高产菌株Streptomyces avermitilisAV-326及其出发菌株Streptomyces avermitilisAV-95为材料,对基因SAV-940(aveC)和SAV-4584(wblA)进行了克隆测序和比对分析,并进一步应用实时荧光定量PCR(RT-qPCR)技术对两个菌株中这两个基因在不同发酵时期(36、48、120和240 h)的相对表达量进行了定量检测和比较。结果显示,在两个菌株中,基因aveC和wblA各自编码的氨基酸序列完全相同;但两个基因表现出不同的表达模式:在所检测的4个时间点,高产菌株中,基因aveC的相对表达量均高于出发菌株,而基因wblA的相对表达量在阿维菌素合成阶段均显著低于出发菌株。In this research,we cloned and sequenced 2 genes[SAV-940（aveC）and SAV-4584（wblA）]which may closely relate to Avermectin production,in both Avermectin overproduction strainStreptomyces avermitilisAV-326 and the initial strain Streptomyces avermitilis AV-95 which the overproduction strain resulted from.In addition,Real-time quantitative PCR was applied to detect the relative expression levels of the 2 genes,at 4 time points（36 h,48 h,120 h and 240 h,respectively）in the fermentation process.Results showed that amino acids sequencees respectively coded by the 2 genes were exactly the same in both two strains;but expression levels of geneaveCwere obviously higher in the high performance strain than in the initial one at all four tested time points,while those of genewblAwere remarkably higher in the initial strain during Avermectin synthesis period.

关 键 词：阿维菌素 阿维链霉菌 序列分析 实时荧光定量PCR 表达模式 

分 类 号：Q933[生物学—微生物学]