芩花口服液抗鸡新城疫病毒体外研究  

Anti-Newcastle Disease Virus of Qinhua Oral Liquid in vitro

作　　者：段凤娟[1] 李继昌[1] 赵高伟[1] 蒋红[1] 孙美成[1] 王凤霞[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国家禽》2013年第17期18-20,共3页China Poultry

基　　金：黑龙江省博士后科研基金资助项目(LBH-Q05026)

摘　　要：为研发抗新城疫病毒(NDV)新药,将黄芩、金银花、前胡、甘草等中药制成的芩花口服液稀释至安全范围浓度,在鸡胚成纤维细胞(CEF)培养系统中进行抗NDV感染试验。用MTT法测定NDV感染CEF能力的变化。结果显示:先加芩花口服液后接种NDV、先接种NDV后加芩花口服液、芩花口服液和NDV同时加入,芩花口服液浓度为0.0625、0.125、0.25、0.50、1.0、2.0 mg/mL时均有显著抗NDV感染CEF作用。此试验初步为寻求抗NDV药物提供一条新途径。The purpose of this experiment was to develop a new anti-Newcastle disease virus（NDV）drug.Qinhua oral liquid,which was made of radix scutellariae,flos lonicerae,peucedanum,licorice et al,was diluted into the safety concentrations,and their anti-infective effects on NDV were studied in chicken embryo cell（CEF）.The effects of Qinhua oral liquid on CEF infectivity of NDV were assayed by MTT method.Results showed that pre-added or post-added or simultaneous added Qinhua oral liquid of 0.0625,0.125,0.25,0.50,1.0,2.0 mg/mL could be anti-infective significantly.This experiment provided a new way for anti-NDV drug.

关 键 词：芩花口服液 鸡胚成纤维细胞 新城疫病毒 抗感染 

分 类 号：S858.315.3[农业科学—临床兽医学]