HPLC法同时测定草珊瑚中7个活性成分的含量  

Simultaneous determination of seven active constituents in Sarcandra glabra by HPLC

作　　者：林培玲[1] 曾建伟[2] 魏艺聪[1] 丁春花[1] 梁一池[1] 

机构地区：[1]福建中医药大学药学院,福州350122 [2]福建中医药大学中西医结合研究院,福州350122

出　　处：《药物分析杂志》2013年第9期1512-1517,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：福建省科技厅重点项目(2011Y4005);福建省科技厅闽澳合作项目(2011I0003);福建省教育厅项目(JA11138)

摘　　要：目的:建立HPLC法同时测定草珊瑚药材中反丁烯二酸、新绿原酸、绿原酸、隐绿原酸、异嗪皮啶、落新妇苷、迷迭香酸含量。方法:采用Agilent Zorbax Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相A为乙腈-甲醇(1∶9),流动相B为0.5%的甲酸水溶液,梯度洗脱(0～20 min,20%A;20～30 mim,20%A→30%A;30～60 min,30%A),流速为0.8mL·min–1,检测波长为228 nm(检测反丁烯二酸)、344 nm(检测新绿原酸、绿原酸、隐绿原酸、异嗪皮啶、落新妇苷、迷迭香酸),柱温为40℃。结果:反丁烯二酸、新绿原酸、绿原酸、隐绿原酸、异嗪皮啶、落新妇苷、迷迭香酸浓度分别在24.12～180.90、1.01～101.00、1.75～175.04、2.19～219.20、1.27～127.20、1.57～157.08、2.93～193.28μg·mL-1范围内与峰面积呈良好的线性关系,平均加样回收率(n=6)均在93.92%～103.0%范围内,RSD均小于3.0%。结论:该方法准确,灵敏度高,重复性好,适用于草珊瑚药材中7个有效成分的含量测定。Objective ： To develop an HPLC method for simultaneous determination of seven main bioactive constitu- ents including fumaric acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenin acid, isofraxidin, astilbin, rosmarinic acid in the whole plant of Sarcandra glabra （Thunb.） Nakai. Methods： An Agilent Zorbax Eclipse XDB -C18 column （250 mm×4. 6 ram, 5 μm） was adopted and the column temperature was at 40 ℃. The mobile phase consisted of a water solution containing 0.5% formic acid and acetonitrile - methanol （ 1 ： 9） in gradient mode [ 0 -20 min ,20% acetonitrile - methanol （ 1：9 ） ;20 - 30 mira, 20%→30% acetonitrile - methanol （ 1：9 ） ;30 -60 min ,30% acetonitrile -methanol （1： 9）], the flow rate was 0. 8 mL . min-1 ,the detection wavelength was set at 228 nm for detecting fumaric acid and 344 nm for detecting neochlorogenic acid, chlorogenic acid, cryptochloro- genin acid, isofraxidin, astilbin, and rosmarinic acid. Results： The good linearity with peak areas was achieved in the range of 24. 12 - 180.90μg . mL-1 for fumaric acid, 1.01 - 101.00 μg . mL-1 for neochlorogenic acid, 1.75 - 175.04 μg . mL-1 for chlorogenic acid, 2. 19 - 219.20 μg . mL-1 for cryptochlorogenin acid, 1.27 - 127.20μg. mL-1 for isofraxidin, 1.57 - 157.08 μg . mL-1 for astilbin, 2. 93 - 193.2μg . mL-lfor rosmarinic acid. The average recoveries（ n = 6） were 93.92% - 103.0% with RSD less than 3.0%. Conclusion： The meth- od developed is accurate, sensitive, reproducible, which can be used for the determination of the seven bioactive constituents in the whole plant of S. glabra.

关 键 词：草珊瑚 肿节风 活性成分 有机酸 反丁烯二酸 新绿原酸 绿原酸 隐绿原酸 异嗪皮啶 落新妇苷 迷迭香酸 高效液 相色谱 

分 类 号：R917[医药卫生—药物分析学]