MEKC-DAD同时测定黄芩中6个黄酮类成分的含量  

Simultaneous determination of six flavonoids in Scutellariae Radix by MEKC-DAD

作　　者：韩乐[1] 陈巧霞[1] 刘训红[1] 周逸芝[1] 李俊松[1] 蔡宝昌[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,南京210023

出　　处：《药物分析杂志》2013年第9期1525-1530,1550,共7页Chinese Journal of Pharmaceutical Analysis

基　　金：江苏省中药炮制重点实验室开放式课题(ZYPZ007);江苏高校优势学科建设工程资助项目(ysxk-2010)

摘　　要：目的:建立胶束电动毛细管色谱二极管阵列检测法(MEKC-DAD)同时测定不同批次黄芩及其炮制品中黄芩苷、黄芩素、汉黄芩苷、汉黄芩素、千层纸素A及芹菜素含量。方法:以40 mmol·L-1磷酸氢二钠-15 mmol·L-1硼砂-40 mmol·L-1十二烷基硫酸钠-15%乙腈-7.5%丙醇(pH 8.4)为电泳介质,未涂渍标准熔融石英毛细管(75μm×64.5 cm,有效长度56cm)为分离通道,分离电压为20 kV,检测波长为280 nm,毛细管温度为25℃,压力进样为5 kPa×6 s。结果:6个黄酮的浓度与峰面积的线性关系良好(r>0.9990);加样回收率为96.20%～103.6%。用此法测定了黄芩中6个黄酮类成分的含量,得到满意结果。结论:该方法简单、准确,重复性较好,可用于黄芩药材内在质量的评价和控制。Objective：To establish a method for simultaneous determination of baicalin, baicalein, wogonoside, wogonin, oroxylin A and apigenin in Scutellariae Radix of different batches and its processed products by micellar electrokinetic chromatography with diode array detection （MEKC -DAD ）. Methods： The buffer solution （pH 8.4） of 40 mmol . L-1 sodium hydrogen phosphate -15 mmol . L-1 sodium borate -40 mmol . L-1 SDS -15% acetonitrile- 7.5% propyl alcohol was used as the electrophoretic medium and the standard uncoated fused silica capillary （75μm . 64.5 cm, 56 cm of effective length） was used as the separation pathway. The separation voltage was 20 kV with a detection wavelength of 280 nm ; the column temperature was maintained at 25 ~C, and the sam- ple was injected at 5 kPa, 6 s. Results： The above- mentioned six flavonoids showed good linearity（r 〉 0. 9990） in the range of the tested concentration, and the average recoveries of the method were between 96.20% - 103.6%. Conclusion： The method is simple, accurate and reproducible, and can be used for quality control and evaluation of Scutellariae Radix.

关 键 词：胶束电动毛细管色谱二极管阵列检测法 黄芩 黄芩苷 黄芩素 汉黄芩苷 汉黄芩素 千层纸素A 芹菜素 中药质量评价和控制 

分 类 号：R917[医药卫生—药物分析学]