UFLC法同时测定新型香料中2个毒品成分的含量  

UFLC simultaneous determination of two narcotics compositions in a novel spice

作　　者：徐鹏[1,2] 林文斯[1] 李晓娜[1] 刘克林[2] 凌笑梅[1] 卢炜[1] 

机构地区：[1]北京大学药学院,北京100191 [2]公安部物证鉴定中心,北京100038

出　　处：《药物分析杂志》2013年第9期1538-1541,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立超快速液相色谱法同时测定新型香料中1-丁基-3-(1-萘甲酰基)吲哚(JWH-073)和1-戊基-3-(1-萘甲酰基)吲哚(JWH-018)的含量。方法:采用超快速液相色谱法。色谱柱:Shim-pack XR-ODSⅡ柱(2.0 mm×75mm,2.2μm);流动相:乙腈-水,梯度洗脱(0～10 min,65%→85%乙腈;10～11 min,85%→65%乙腈;11～15 min,65%乙腈);流速:0.25 mL·min-1;检测波长:280 nm,柱温:35℃,进样量:5μL。外标法定量测定JWH-073与JWH-018这2个组分的含量。结果:JWH-073与JWH-018的线性关系良好,平均回收率(n=9)分别为98.58%、98.72%。结论:本法线性良好,灵敏度高,重复性好,简便可行,可用于同时测定新型香料中JWH-073与JWH-018的含量。Objective：To establish an UFLC method for simultaneous determination of two compositions （JWH - 073, JWH -018） in a new type spice. Methods：A UFLC method was applied with a Shim - pack XR - ODS Ⅱ chromatographic column （2.0 mm × 75mm, 2. 2 μm）, the mobile phase consisted of acetonitrile and water, elu- ted in gradient mode（0 - 10 min, 65%→85% acetonitrile; 10 - 11 min, 85%→65% acetonitrile; 11 - 15 min, 65 % aeetonitrile）. The mobile phase was pumped at a flow rate of O. 25 mL . min-1 The UV detection wavelength was set at 280 nm. The column temperature was controlled at 35 ℃. The injection volume was 5 μL. The contents of JWH- 073 and JWH -018 in the new type spice were calculated by external standardization method. Results： There were good linear relationships for JWH -073 and JWH -018. The average recoveries （n = 9 ） were 98.58% , 98.72%, respectively. Conclusion：The established UFLC method is simple, sensitive, reproducible and feasible for simultaneous determination of the two compositions, JWH-073 and JWH- 018 in the new type spice.

关 键 词：新型香料 1-丁基-3-（1-萘甲酰基）吲哚 1-戊基-3-（1-萘甲酰基）吲哚 合成大麻素含量测定 超快速液相 色谱法 外标法 

分 类 号：R917[医药卫生—药物分析学]