Ezrin和NEPH1在多柔比星肾病大鼠肾组织中的表达及泼尼松对其表达的影响  

Effect of prednisone on expression of Ezrin and NEPH1 in rats with Adriamycin-induced nephrosis

作　　者：王娟[1] 张碧丽[2] 

机构地区：[1]天津医科大学研究生院,300070 [2]天津市儿童医院肾内科

出　　处：《中华实用儿科临床杂志》2013年第17期1323-1326,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的 通过观察足蛋白Ezrin和NEPH1在多柔比星肾病大鼠肾组织中的表达及泼尼松对其表达的影响,探讨蛋白尿产生的机制及泼尼松对肾脏的保护作用.方法 将24只大鼠随机分为空白对照组、模型组、泼尼松干预组,通过分次尾静脉注射法建立多柔比星肾病大鼠模型,监测第4、8周时大鼠血生化及24 h尿蛋白变化.应用免疫组织化学两步法观察肾组织中足蛋白Ezrin和NEPH1表达的变化.结果 实验第4周,与空白对照组比较,模型组与泼尼松干预组均出现大量蛋白尿、低清蛋白血症和高脂血症（P均＜0.01）,提示模型建立成功；实验第8周,与模型组比较,泼尼松干预组24 h尿蛋白、总胆固醇、血肌酐水平显著降低（P均＜0.01）,清蛋白、总蛋白、内生肌酐清除率均增高（P均＜0.05）；免疫组织化学示模型组Ezrin和NEPH1的表达较空白对照组显著降低（P均＜0.01）,泼尼松干预组Ezrin和NEPH1的表达较模型组显著增加（P均＜0.01）,足蛋白Ezrin、NEPH1的表达与24 h尿蛋白均呈负相关（r=-0.838、-0.884,P均＜0.01）.结论 足蛋白Ezrin和NEPH1表达下降可能是多柔比星肾病大鼠蛋白尿产生的机制之一.泼尼松可能通过增加Ezrin和NEPH1的表达减少尿蛋白,起到保护肾脏的作用.Objective To explore the mechanism of proteinuria and renal protection of prednisone by observing the expressions of Ezrin and NEPH1 in rats with Adriamycin （ADR）-induced nephrosis.Methods The 24 rats were divided into 3 groups,which were control group,model group and prednisone group.ADR model was induced by a tail intravenous injection of ADR for 2 times.Serum index and 24 h urinary protein were measured in 4 and 8 weeks.The expressions of Ezrin and NEPH1 in glomerulus were evaluated by using two-step immunohistochemistry respectively.Results Compared with control group,heavy proteinuria,hypoalbuminemia,hyperlipemia were observed in 4 weeks in the model group and the prednisone group which indicated that the models were successfully established.Compared with model group,in 8 weeks,24 h urinary protein,total cholesterol and serum creatinine in prednisone group were significantly decreased（all P ＜ 0.0 l）,while albumin,total protein and endogenous creatinine clearance rate were significantly increased（all P ＜ 0.05） ;The expressions of Ezrin and NEPH1 in model group were significantly decreased compared with control group（all P ＜ 0.01）.The expressions of Ezrin and NEPH1 in prednisone group were significantly increased compared with model group（all P ＜0.01）.The expressions of Ezrin and NEPH1 were negatively related to proteinuria（r =-0.838,-0.884,all P ＜ 0.01）.Conclusions The declined expressions of Ezrin and NEPH1 in rats with ADR-induced nephrosis may be one of mechanisms of proteinuria.Prednisone can reduce the proteinuria and relieve the renal pathological damage by improving the expression of Ezrin and NEPH1.

关 键 词：多柔比星肾病 肾病综合征 泼尼松 EZRIN NEPH1 

分 类 号：R965[医药卫生—药理学]