华蟾素诱导人胃癌BGC-823细胞miRNA表达变化的实验研究  

miRNA expression of BGC-823 cell induced by cinobufacin

作　　者：周荣平[1,2] 陈刚[2] 沈志力[2] 潘立群[1] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210046 [2]南京医科大学附属江宁医院,江苏南京211100

出　　处：《中成药》2013年第9期1842-1846,共5页Chinese Traditional Patent Medicine

基　　金：卫生部医药卫生科技发展项目(W2012FZ146)

摘　　要：目的研究华蟾素对人胃癌BGC-823细胞MicroRNA(miRNA)表达谱的影响。方法应用华蟾素处理胃癌细胞系BGC-823细胞,以MTT法确定细胞增殖受到抑制后,基因芯片方法分析BGC-823细胞在药物处理前后miRNA表达谱的变化,qRT-PCR法验证miRNA的表达水平。结果随华蟾素质量浓度增加及作用时间的延长,华蟾素对人胃癌细胞BGC-823细胞增殖抑制率相应增加,具有时效和量效关系。人胃癌细胞BGC-823中,华蟾素诱导了33个miR-NA的表达上调,12个miRNA的表达下调。qRT-PCR验证华蟾素诱导的miR-29a和miR-26a的表达上调的结果。结论华蟾素可能通过诱导miRNA表达谱的改变参与抑制人胃癌细胞增殖作用。AIM To study the influence of cinobufacin on the expression profile of microRNA（miRNA） of the human gastric adencarcinoma cell BGC-823.METHODS Cytotoxicity of cinobufacin against BGC823 cells was measured by MTT assay.The miRNA expression profile of the BGC-823 cells was analyzed by a miRNA array and quantitative real-time PCR. RESULTS Cinobufacin gradually increased the cell proliferation inhibition rate of BGC-823 in a time-and dose-dependent manner with the increase of concentration and prolongation of action time.Moreover,cinobufacin influenced the miRNA expression pattern in BGC-823 cells with up-regulating 33 miRNAs and down-regulating 12 miRNAs in the cells.The up-regulations of miR-29a and miR-26a,verified by Q-PCR,were found to be consistent with the results of qRt-PCR.CONCLUSION Differentiation of BGC-823 cell can be induced with cinobufacin,and miRNA expression may play a major role in inhibiting the proliferation.

关 键 词：华蟾素 胃癌 增殖 MIRNA 

分 类 号：R285.5[医药卫生—中药学]