大豆素与葛根素抑制LPS诱导RAW264.7细胞NO产生的活性比较  

Comparison of Inhibitory Activity between Daidzein and Puerarin on LPS Induced NO Production in RAW264.7 Cells

作　　者：张明 王迪 万义增[2] 艾浩[2] 李军祥[3] 李健[4] 

机构地区：[1]锦市第二人民医院,盘锦市1240102 [2]辽宁医学院附属三院,锦州121000 [3]北京中医药大学东方医院,北京100078 [4]北京中医药大学基础医学院,北京100029

出　　处：《世界科学技术-中医药现代化》2013年第4期648-652,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：北京中医药大学自主选题项目(JYBZZ-JS004):基于“氧化损伤”学说探讨雷公藤多苷片对正常及CIA大鼠肝损伤的分子机制及姜黄素的调节作用,负责人:李健;辽宁省科学技术厅科学技术计划项目(2009225010-47):LPS诱导酒精性肝病大鼠门脉高压分子机制及姜黄素的调节作用,负责人:万义增

摘　　要：目的:比较分析大豆素与葛根素体外抗氧化及抑制NO、NOS活性机制。方法:用1μg·mL-1LPS诱导体外培养的RAW264.7细胞,制备呼吸爆发、自由基反应模型。采用MTT法检测大豆素及葛根素的细胞毒性;采用DCFH-DA探针及激光共聚焦显微镜观察被试药的抗氧化能力;采用Griess法检测细胞培养体系中NO水平;采用化学比色法测定细胞内T-NOS含量。结果:大豆素与葛根素均能显著抑制LPS诱导RAW264.7细胞NO释放及T-NOS表达。结论:大豆素与葛根素的抗氧自由基活性接近,有望作为葛根素的替代品使用。This study was aimed to compare the activation of daidzein and puerarin on antioxidation,NO and NOS suppression in vitro.The RAW264.7 cell line was used to prepare radical reaction model induced by LPS（1 μg/mL）.MTT method was adopted to detect cytotoxicity of daidzein and puerarin.The DCFH-DA probe and confocal microscopy were used to examine the antioxidant ability of daidzein and puerarin.Griess reagent was adopted to test the NO level in the culture medium.And chemical colorimetry was used to detect the content in RAW264.6 cells.The results showed that daidzein and puerarin can significantly suppress the NO and T-NOS expression in RAW264.7 cells induced by LPS.It was concluded that there was no difference on the activation of antioxidant free radical between daidzein and puerarin.In this regard,daidzein can be the used as substitutes of puerarin.

关 键 词：大豆素 葛根素 RAW264 7 NO 

分 类 号：R285[医药卫生—中药学]