补肾活血通淋方对良性前列腺增生症大鼠模型Ki-67及凋亡小体的影响  

Bushen-Huoxue-Tonglin Formula on Benign Prostatic Hyperplasia in Rat Model of Ki-67 and Apoptotic Effects

作　　者：孙自学[1] 陈建设[1] 周东[1] 

机构地区：[1]河南省中医院,郑州450002

出　　处：《世界科学技术-中医药现代化》2013年第4期653-658,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：河南省教育厅河南省高校科技创新人才支撑计划(2011HASTIT019):补肾活血通淋方对良性前列腺增生症大鼠模型bFGF;Caspase-3;Bcl-2影响的研究;负责人:陈建设;郑州市科技局郑州市科技创新团队(121PCXTD522):基于肾主气化理论研究益肾活血清利方对前列腺增生大鼠TGF-β1和bFGF的影响;负责人:孙自学

摘　　要：目的:观察补肾活血通淋方对良性前列腺增生症大鼠模型Ki-67、凋亡小体的影响。方法:72只Wistar大鼠随机分为6组,即假手术组、模型组、保列治组、补肾活血通淋方低、中、高剂量组。除假手术组外,余组摘除大鼠双侧睾丸后连续注射丙酸睾丸酮(5 mg·kg-1)30天建立模型,造模同时,给药组灌胃给药,给药剂量:保列治组(0.8 mg·kg-1)、补肾活血通淋方低、中、高剂量组(7.5、15.0、22.5 g·kg-1),每天1次,连续30天。采用免疫组化法测定Ki-67的阳性平均灰度值,采用TUNEL法测定凋亡小体的表达率。结果:与模型组比较,各给药组Ki-67表达均降低(P<0.05);与保列治组、补肾活血通淋方低剂量组比较,补肾活血通淋方高、中剂量组Ki-67表达降低(P<0.05),与中剂量组比较,高剂量Ki-67表达降低(P<0.05);与模型组比较,各治疗组均能上调前列腺增生中凋亡小体的表达(P<0.05)。其中补肾活血通淋方高剂量组凋亡小体表达率高于中、低剂量组、保列治组(P<0.05)。结论:补肾活血通淋方可以降低良性前列腺增生症大鼠Ki-67的表达,提高细胞凋亡率,为临床治疗良性前列腺增生症提供了客观依据。This study was aimed to observe the effect of Bushen-Huoxue-Tonglin formula（BSHXTLF） on Ki-67 and apoptotic bodies in benign prostatic hyperplasia（BPH） rats.A total of 72 Wistar rats were randomly divided into 6 groups,which were the sham operation group,model group,Proscar group（0.8 mg·kg^-1）,BSHXTLF groups with low,middle and high dose（7.5,15,22.5 g·kg-1）.Except the sham operation group,the BPH model was established by injecting testosterone propionate（5 mg·kg-1） for 30 days after removing both testicles in the rats.The drugs were administrated once a day for 30 days at the same time.The immunohistochemical and TUNEL methods were used to determine Ki-67 and apoptotic bodies,respectively.The results showed that compared with the model group,the expression of Ki-67 in treatment groups was decreased（P〈0.05）.Compared with Proscar group and low-dose BSHXTLF group,the expression of Ki-67 in the middle-and high-dose BSHXTLF group was decreased（P〈0.05）.Compared with the middle-dose BSHXTLF group,the expression of Ki-67 in the high-dose BSHXTLF group was decreased（P〈0.05）.Compared with the model group,the expression of apoptotic bodies in each treatment group was increased（P〈0.05）.Compared with Proscar group,low-dose and middledose BSHXTLF group,the expression of apoptotic bodies in the high-dose BSHXTLF group was increased（P〈0.05）.It was concluded that BSHXTLF may decrease the expression of Ki-67 and increase the expression of apoptotic bodies in BPH rats.These results provided objective evidences for clinical treatment of BPH.

关 键 词：补肾活血通淋方 KI-67 凋亡小体 良性前列腺增生症 

分 类 号：R697.32[医药卫生—泌尿科学]