等温微量量热法研究石油污染对土壤微生物活性的影响  

Microcalorimetric investigation of effects of petroleum pullution on soil microbial activity

作　　者：佘文文[1,2] 姚俊[1] 王飞[1] 蔡珉敏[1] 王静伟[1] 宋长顺[1] Brunello Ceccanti Serena Doni 

机构地区：[1]北京科技大学土木与环境工程学院,国家环境与能源国际合作基地,北京100083 [2]珠海市德莱环保科技有限公司,珠海519070 [3]生态系统研究所(ISE),意大利国家研究委员会(CNR)

出　　处：《环境工程学报》2013年第9期3624-3628,共5页Chinese Journal of Environmental Engineering

基　　金：国家杰出青年科学基金资助项目(40925010);国家自然科学基金国际合作重大项目(40920134003);国家自然科学基金资助项目(40873060);科技部国际合作重点项目(2011DFA00120;2009DFA92830;2010DFA12780)

摘　　要：利用TAMⅢ等温微量量热仪研究不同浓度的石油污染对土壤微生物活性的影响。对大港油田不同石油污染程度的土壤样品(3683-5-1、西51-5、西15-14和8-13-6H-1)以及实验室配置的短时间内石油污染土壤(空白、0.5%、1.5%、2.5%和4.0%)进行了微量热检测。含油量升高,总放热量升高,生长速率常数降低,峰值功率先升高后降低,达到最大峰值功率的时间随着含油量的升高而延长。结果表明,石油污染会影响土壤微生物的活性,并且在低浓度时促进微生物的生长,石油浓度高时会抑制土壤中微生物的生长代谢。TAM Ⅲ multi-channel calorimetry was applied to study the effect of different concentrations petroleum on soil microbial activity.The soils samples from different wells of Dagang Oil field（3683-5-1,West 515,West 15-14,8-13-6H-1） were detected.At the same time,serials of soils with different concentrations of petroleum（control,0.5%,1.5%,2.5%,4.0%） were prepared to study their short-time effect on microbial community activity.As the oil content increased,the total heat evolution increased,the microbial growth rate constant decreased,the peak-heat output powers increased first and then started to decline when reached a certain concentration,and the times of the peak delayed after crude petroleum added.The results indicate that the soil microbial activity was promoted at the lower concentration but then inhibited accordingly with increasing concentrations of petroleum.

关 键 词：微量热 土壤微生物 石油 酶活 

分 类 号：Q938.13[生物学—微生物学] X53[环境科学与工程—环境工程]