大鼠局灶性脑挫裂伤不同损伤区域水通道蛋白4表达变化的实验研究  

The AQP4Expression Changes of Different Areas of the Controlled Cortical Impact Injury in Rats

作　　者：赵旭[1] 柳云恩[2] 刘恩智[3] 张海松[3] 张兴[3] 雷伟[3] 刘宏波[3] 李宾[3] 宋振全[3] 

机构地区：[1]辽宁医学院,锦州121000 [2]沈阳军区总医院全军重症战创伤中心实验室,沈阳110840 [3]沈阳军区总医院神经外科,沈阳110840

出　　处：《成都医学院学报》2013年第4期403-407,共5页Journal of Chengdu Medical College

基　　金：辽宁省科技攻关课题项目(NO:2009225018)

摘　　要：目的探索水孔通道蛋白4(AQP4)在局灶性脑挫裂伤(CCII)不同损伤区域表达变化,及其对创伤性脑水肿血脑屏障(BBB)的影响。方法雄性成年Wistar大鼠,随机分为假手术组和CCII组,其中CCII组分为损伤中心组和损伤外周组。参照改良Feeney’s自由落体硬膜外撞击法制作CCII模型。结果损伤中心组和损伤周围组脑组织含水量明显高于假手术组;免疫组织化学结果显示损伤中心组在各检测时间点的AQP4含量和伊文思蓝(EB)含量均明显高于假手术组和损伤周围组,差异有统计学意义(P<0.05)。结论 CCII后AQP4蛋白的表达变化趋势和BBB的通透性相一致;损伤中心AQP4含量与损伤周围AQP4含量存在差异,这与相应区域的脑组织水肿类型相关。脑挫裂伤外周水肿带AQP4含量是降低的,损伤中心部位AQP4含量是升高的。损伤中心以血管源性脑水肿为主,脑挫裂伤外周水肿带以细胞毒性脑水肿为主。Objective To investigate the changes of aquaporin4 （AQP4） expression in controlled cortical impact injury（CCII） of different areas, and the impact on the blood brain barrier （BBB） of traumatic brain edema. Methods Male adult Wistar rats were randomly divided into sham operation group and the CCII group, the CCII group was divided into the centre region and peripheral region group. CCII model was established according to the Feeney＇s method. Results The water contents of brain in the centre region group and the peripheral region group were significantly higher than the sham operation group. The immunohistochemical results showed that the AQP4 expression and the BBB permeability of the centre region group were significantly higher than the sham operation group and the peripheral region group （P〈0.05）. Conclusion The expression of AQP4 and the permeability of BBB change after CCII; The expression of AQP4 between the centre region and pericontusional zone is different, corresponding to the brain edema area and type. The expression of AQP4 in pericontusional zone is lower, while in the centre region is higher. The brain edema type in the centre region is predominantly vasogenic edema, and in pericontusional zone is predominantly cytotoxic brain edema.

关 键 词：水通道蛋白4 局灶性脑挫裂伤 损伤中心 脑挫裂伤外周水肿带 

分 类 号：R651.15[医药卫生—外科学]