复合RT-PCR检测猪繁殖与呼吸综合征和猪瘟及猪流感等3种病毒方法的建立  

Establishment of Multiplex Reverse Transcription Polymerase Chain Reaction for the Detection of Porcine Reproductive and Respiratory Syndrome Virus and Classical Swine Fever Virus and Swine Influenza Virus

作　　者：夏俊[1] 石进纯[1,2] 成进[1] 张美玲[1,2] 陆桂丽[1] 汪萍[1] 

机构地区：[1]新疆畜牧科学院兽医研究所,新疆乌鲁木齐830000 [2]新疆农业大学

出　　处：《现代农业科技》2013年第17期275-277,共3页Modern Agricultural Science and Technology

基　　金：新疆科技厅科技攻关项目"新疆养猪业三种主要疫病防控技术研究与应用"(201031106)

摘　　要：为了快速准确地对猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV)和猪流感病毒(SIV)进行诊断与鉴别诊断,特进行试验。根据GenBank上已发表的PRRSV的ORF7基因序列、CSFV的C基因序列和SIV的M基因序列,设计合成了3对特异引物,开展RTPCR检测方法的研究。分别建立了PRRSV、CSFV和SIV的单项RT-PCR诊断方法,并最终建立了PRRSV、CSFV和SIV的复合RT-PCR诊断方法,可分别及同时扩增PRRSV 430 bp、CSFV 775 bp和SIV 225 bp的特异性片段。试验证明该方法适用于PRRSV、CSFV和SIV的诊断及鉴别诊断。In order to diagnose and differential diagnose for PRRSV ,CSFV and SIV ,this experiment was carried out.Based on the ORF7 gene sequences of PRRSV, C gene sequences of CSFV and M gene sequences of SIV obtained from GenBank,three pairs of primers were designed and used for RT-PCR.Single RT-PCR assays for diagnosing PRRSV, CSFV and SIV were established, the multiplex RT-PCR was also developed in the end, the fragments of 430 bp for PRRSV ,775 bp for CSFV and 225 bp for SIV could be amplified in single RT-PCR and a multiplex RT-PCR assay.According to the test,the RT-PCR assay was suit to diagnose and differential diagnose for PRRSV, CSFV and SIV.

关 键 词：复合RT-PCR 检测 猪繁殖与呼吸综合征病毒 猪瘟病毒 猪流感病毒 

分 类 号：S852.65[农业科学—基础兽医学]