牛病毒性腹泻病毒BVDV2/JZ05-2毒株牛感染模型建立  

Bovine Viral Diarrhea Virus BVDV2/JZ05- 2 Strain Infection Modeling

作　　者：冯卓[1] 刘艳环[1] 朱言柱[1] 李海涛[1] 钟宏鹏 王坤[1] 张润祥[1] 苗利光[1] 

机构地区：[1]中国农业科学院特产研究所,长春130112 [2]吉林省集安市清河镇畜牧站,吉林集安134214

出　　处：《特产研究》2013年第3期27-30,共4页Special Wild Economic Animal and Plant Research

基　　金：吉林省科技发展计划项目(20100219)

摘　　要：试验用4～6月龄健康牛22头，共分5组，4个试验组5头/组，对照组2头。各试验组分别鼻内接种强毒107 FAID50/mL、106 FAID50/mL、105 FAID50/mL、104 FAID50/mL ，4mL/头，2mL/鼻孔。对照组鼻内接种MDBK细胞培养液冻融物，4mL/头，2mL/鼻孔。试验动物接种强毒后，每天观察实验动物的反应，包括精神状态、体温、食欲、黏膜颜色、呼吸频率、咳嗽、鼻腔分泌物及腹泻等；每天采集鼻拭子用于病毒分离。攻毒前2d、0d ，攻毒后隔天采血用于白细胞计数和病毒分离；试验于病毒接种后14d结束。试验过程中如有动物死亡，对死亡动物进行病理学检查。结果，106.6 FAID50/头BVDV2/JZ05-2攻击4～9月龄的牛，可引起被攻击牛有规律地体温升高和白细胞数量下降，同时可以从鼻拭子中分离到病毒。以这些指标作为感染模型可以有效地评价BVDV2疫苗免疫效果。The aim of this experiment was to build bovine viral diarrhea virus BVDV 2/JZ05-2 strain infection model .Twenty two healthy cattle （4~6 months old ） were divided into 5 groups .In four experimental groups ,each group had 5 cattle .The control group had 2 cattle .The con-centrations of the BVDV2/JZ05-2 strain used in the experimental groups were 107 FAID50/mL ,106 FAID50/mL ,105 FAID50/mL ,104 FAID50/mL ,respectively .In control group ,the cattle were given MDBK nutrient solution .The volume of the sample was 4 mL through intranasal in dif-ferent groups .After inoculated the strong virus ,the clinical symptom was recorded everyday .The clinical symptom included psychosis ,tempera-ture ,appetite ,mucosa colour ,respiratory rate ,cough ,nasal cavity secreta and diarrhea .Nose swab was collected to separate the strong virus ev-eryday .The blood was obtained to determine white blood cell count and separate virus in -2 day ,0 day and 2 day .After the virus was inoculat-ed for 14 days ,the experiment was finished .If the dead cattle were found ,pathological examination should be done .The results showed that the dose of 106.6 FAID50/mL could regularly raise the temperature and reduce the count of white blood cell .In addition ,the virus was obtained from the nose swab .It indicates that the bovine viral diarrhea virus BVDV 2/JZ05-2 strain infection model was successfully built .

关 键 词：牛病毒性腹泻病毒 感染模型 免疫 

分 类 号：S852.653[农业科学—基础兽医学]