鸡ApoA5基因的多态性与胆固醇浓度和繁殖性状的关联分析  

Polymorphisms of Chicken ApoA5 Gene and Their Associations with Cholesterol Content and Reproductive Traits

作　　者：杜晓惠[1] 熊婷[1] 刘俊莹[1] 张怀勇[1] 万火福[1] 朱庆[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014

出　　处：《中国农业科学》2013年第17期3704-3711,共8页Scientia Agricultura Sinica

基　　金：国家蛋鸡产业技术体系(CARS-41);四川省教育厅重点项目(00220103)

摘　　要：【目的】探究ApoA5基因的多态性与胆固醇浓度和繁殖性状之间的关系,为平衡胆固醇浓度与繁殖性状间的关系奠定基础。【方法】试验共记录了434只SD母鸡的繁殖性能、300 d时的血清胆固醇浓度(SCC)和蛋黄胆固醇浓度(YCC),采用PCR-SSCP及PCR-RFLP技术,筛查到ApoA5基因的6个新的SNPs并进行关联分析。【结果】单个SNP的效应比较集中在开产日龄(AFE)和SCC上;由位置靠近、效应一致的SNP2、SNP4和SNP5构建的单倍型,关联分析结果大致与单个SNP相吻合,但更突显了各性状在单倍型组合间的差异,SCC和YCC都较高的H5H8、H4H8、H5H6、H6H6单倍型组合具有较好的繁殖性能,SCC和YCC都较低的H1H5、H1H6和H3H8组合则相反。【结论】ApoA5基因的单倍型SNP2-4-5能够揭示各组合在胆固醇及各繁殖性状间的差异,可以作为提高繁殖性能的分子标记。[Objective] To balance the relationship between cholesterol concentration and reproductive traits, the associations among the traits of cholesterol and reproduction and polymorphisms of chicken ApoA5 gene were studied. [Method] The reproduction traits were recorded and the cholesterol concentrations of serum （SCC） and egg yolk （YCC） were measured in 434 SD hens at 300th day. Through PCR-SSCP and PCR-RFLP analyses, 6 new SNPs were detected in ApoA5 gene and their associations with the traits of cholesterol and reproduction were analyzed. [ Result] The effects of the single SNP mainly focused on the age of first egg （AFE） and on the SCC. The results of association analysis of the haplotype, constructed with SNP2, SNP4 and SNP5 because of their closer location and consistent effects, were roughly accordant with the single SNP, but the trait differences between haplotype combinations were enlarged. The haplotype combinations （H5H8, H4H8, H5H6, H6H6） with higher SCC and YCC had better reproductive performance, and H1H5, H1H6 and H3H8 with lower SCC and YCC were on the contrary. [ Conclusion] Haplotype SNP2-4-5 ofApoA5 gene revealed the differences of cholesterol and reproductive traits among various combinations, and could be used as a molecular markers to improve reproductive traits.

关 键 词：鸡 ApoA5基因 多态性 胆固醇 繁殖性状 关联分析 

分 类 号：S831[农业科学—畜牧学]