采用大孔吸附树脂D-101初步分离提取TJ430菌株发酵液中的抗生素  

Preliminary research on the separation of an agro-antibiotic from the fermentation broth of the Streptomyces strain TJ430 by macroporous absorption resin D-101

作　　者：黄永春[1] 刘红梅[1] 赵鹏[1] 张伟[1] 陈志永[1] 

机构地区：[1]农业部环境保护科研监测所,天津300191

出　　处：《植物保护》2013年第4期85-89,共5页Plant Protection

基　　金：中央级公益性科研院所基本科研业务费专项资金(农业部环境保护科研监测所)自主项目(2012-SZJJ-HYC);天津市自然科学基金项目(12JCYBJC19900)

摘　　要：借助生物活性测定法和高效液相色谱法对发酵液中农用抗生素进行追踪监测,探讨了大孔吸附树脂对菌株TJ430发酵液中农用抗生素的分离纯化条件。经过对比试验发现D-101型大孔吸附树脂的吸附性能最好;最佳吸附时间为120 min;70%丙酮水溶液的解吸附能力最佳;最佳解吸附pH为接近中性条件;最佳解吸附时间为120min。采用D-101型大孔吸附树脂对TJ430菌株发酵液中抗生素进行吸附,在中性条件下经70%丙酮水解吸附,使发酵液中抗生素得到了有效的分离纯化。An agro-antibiotic extract was obtained by bioassay and HPLC. The dynamic adsorption performance of macroporous resin D-101 on the agro-antibiotic from the Streptomyces strain TJ430 fermentation broth was inves tigated. The influences of the adsorption and desorption time, value of pH, and concentration of acetone eluent on extraction were discussed. The results showed that D-101 resin had good adsorption ability for fermentation broth, and the optimum adsorption and desorption conditions were as follows： the adsorption and desorption time was 120 minutes, the desorption concentration of acetone was 70%, and the pH value of desorption was 6.0. The antibiotic was refined efficiently after adsorption and desorption procedure.

关 键 词：大孔吸附树脂 农用抗生素 分离提取 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]