乙型肝炎伴早期肝硬化microRNA表达谱及其诊断价值  

MicroRNA expression profiles and its diagnostic value in hepatitis B-related early liver cirrhosis

作　　者：张美殷[1] 魏蓉蓉[1] 彭亮[2] 高志良[2] 黄国良[3] 王辉云[1] 

机构地区：[1]中山大学肿瘤防治中心华南肿瘤学国家重点实验室,广东广州510060 [2]中山大学附属第三医院感染科,广东广州510630 [3]广东医学院东莞分院中美肿瘤研究所,广东东莞523808

出　　处：《检验医学与临床》2013年第16期2069-2071,2073,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨乙型肝炎伴早期肝硬化和乙型肝炎microRNA(miRNA)差异表达谱,寻找具有诊断价值的miRNA标志物。方法利用miRNA芯片技术检测7例乙型肝炎伴早期肝硬化患者和11例乙型肝炎患者肝组织中miRNA的表达水平。应用ROC曲线评价各miRNA的诊断价值。结果与单纯肝炎组织相比,伴早期肝硬化的肝炎组织中表达上调超过1.5倍的miRNA有miR-625-3p、miR-325、miR-596等5种,表达下调超过1.5倍的miRNA有miR-365、miR-362-5p、miR-139-5p等10种。ROC曲线分析结果表明,miR-139-5p诊断乙型肝炎伴早期肝硬化的特异性和敏感性最高。结论乙型肝炎伴早期肝硬化组织和乙型肝炎组织相比有其特异的miRNA表达谱,miR-139-5p可能是诊断乙型肝炎伴早期肝硬化的一个标志物。Objective To compare miRNA expressions between hepatitis B-related early liver cirrhosis and non-cirrhosis tissues to identify miRNA markers for diagnosis of hepatitis B-related early liver cirrhosis.Methods miRNA expression profiling of seven patients with hepatitis B-related early liver cirrhosis and eleven patients with only hepatitis B was performed using miRNA microarray,and diagnostic value of miRNAs was analyzed by receiver operating characteristic（ROC） curve method.Results Fifteen miRNAs were found to be differentially expressed between hepatitis B related early liver cirrhosis and non-cirrhosis tissues,five（miR-625-3p,miR-325,miR-596,miR-141,miR-339-3p） of which were up-regulated and ten（miR-365,miR-362-5p,miR-193b-5p,miR-139-5p and others） of which were down-regulated.ROC curve analysis showed that AUC value of miR-139-5p was 0.994,which could be the best miRNA for distinguishing hepatitis B-related early liver cirrhosis from patients with only hepatitis B.Conclusion Our results demonstrate that there might be a specific miRNA expression profile in hepatitis B-related early liver cirrhosis and miR-139-5p could be a novel and potential biomarker for hepatitis B-related early liver cirrhosis.

关 键 词：MIRNA芯片 MIRNA 乙型肝炎 肝硬化 

分 类 号：R512.62[医药卫生—内科学]