极端嗜盐古菌嗜盐特性研究及在废水处理中的应用  

Study on the Halophilic Characteristics of Halobacteria and Its Application in Wastewater Treatment

作　　者：李晓琳[1,2] 邵坚[1] 田秉晖[2] 辛丽花[2] 

机构地区：[1]华北水利水电大学环境与市政工程学院,河南郑州450011 [2]中国科学院生态环境研究中心,北京100085

出　　处：《安徽农业科学》2013年第18期7917-7919,7922,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金(51178448);水体污染控制与治理科技重大专项(2012ZX07203-003-R05)

摘　　要：[目的]探讨极端嗜盐古菌的生理生化特性、最适生长条件以及对苯酚的降解效果.[方法]以一株盐生盐杆菌SYGJ-1为研究对象,分析了极端嗜盐古菌的生理生化特性以及不同生长条件（盐度、温度、pH）对其生长情况的影响,并探讨了该菌对苯酚的降解效果.[结果]极端嗜盐古菌SYGJ-1的最适盐度为19％,盐度＜17％或者＞25％时,生长量急剧下降;SEM图片表明,菌体在最适盐度时呈饱满短杆状,盐度小于＜15％时菌体呈球状,在盐度大于＞29％时会同时出现球状及长杆状菌体;最适温度为37℃,对数生长期为12～66h;适宜pH范围为6.8 ～9.0;在苯酚浓度＜200 mg/L的培养基中生长良好,此时对苯酚的去除效率约为40％.[结论]极端嗜盐菌可有效降解有机污染物,并且菌体生长量与降解效率呈正相关.[Objective] The study aimed to discuss the physiological and biochemical characteristics,optimal growth conditions and degradation efficiency of haloarchaea to phenol.[Method] Physiological and biochemical characteristics of an extremely halophilic archaea called SYGJ-1 was analyzed firstly,and then the effects of salinity,temperature and pH on its growth were studied,finally its degradation efficiency to phenol was discussed.[Result] The optimal salinity for SYGJ-1 growth is 19％,and there is a sharp decline in growth when salinity is ＜17％ or ＞ 25％.SEM images show that the bacteria shows full short rod as salinity is the optimum; it is spherical when salinity is low than 15％ ; spherical and long rod-shaped bacteria can be found at the same time.The optimum temperature is 37 ℃,and the logarithmic phase is 12-66 h.Suitable pH range is from 6.8 to 9.0.In the medium with phenol concentration of ＜200 mg/L,SYGJ-1 grows well,and the removal rate of phenol is about 40％.[Conclusion] The extreme halophilic bacteria can effectively degrade organic pollutants,and the degradation efficiency of phenol has a positive relation with its growth.

关 键 词：嗜盐古菌 盐生盐杆菌 嗜盐特性 高盐废水 苯酚降解 

分 类 号：S271[农业科学—农业水土工程]