双插管蛙心在离体蛙心灌流实验中的应用  

Application of Double-cannula Method in Isolated Frog Heart Perfusion Experiment

作　　者：王慧平[1] 蒿慧文[1] 肖雪[1] 张扬媚[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《北华大学学报（自然科学版）》2013年第5期557-559,共3页Journal of Beihua University（Natural Science）

基　　金：陕西省省级精品课程(动物生理学)建设项目;西北大学资产设备处2012年实验教学资助项目;西北大学国家级大学生创新实验项目(10697201013)

摘　　要：目的探讨单、双插管蛙心在离体蛙心灌流实验中的应用效果.方法对比两种插管方法对离体蛙心活性及对灌流结果的影响.结果双插管法对离体蛙心活性的影响较小,在灌流实验中使用双插管蛙心,可实现对心输出量和每搏输出量的记录.双插管蛙心还可用于研究蛙心前后负荷对蛙心功能的影响.结论双插管蛙心是更为理想的离体蛙心灌流实验模型.Objective To discuss the application effects of single-cannula method and the double-cannula method in the isolated frog heart perfusion experiment. Method The influence of two intubation methods on isolated frog heart activity and the perfusion resuJts was compared. Results The influence of the douhle-cannula intubation method on isolated frog heart activity was not so significant in contrast to the single-cannula intuhation method. The application of the double-cannula in the isolated heart perfusion experiment could make recording cardiac output and stroke volume become possible, and the method could be used in the study on the influence of the cardiac preload and afterload on the changes of cardiac functions of the heart. Conclusion The isolated frog heart with double-cannula intubation should be a more ideal experiment model used in the in vitro fi-og perfusion experiment.

关 键 词：双插管法 离体蛙心灌流 心脏负荷 心功能 

分 类 号：R331[医药卫生—人体生理学]