瑞舒伐他汀与去氧皮质酮-盐诱导的心肌纤维化  

Rosuvastatin and desoxycorticosterone-salt-induced myocardial fibrosis

作　　者：吴远贵 李谦 马礼坤 

机构地区：[1]安徽省第二人民医院心内科,合肥230001

出　　处：《安徽医学》2013年第9期1273-1276,共4页Anhui Medical Journal

摘　　要：目的通过研究瑞舒伐他汀(ROS)对去氧皮质酮(DOCA)-盐联合诱导的大鼠心肌纤维化的干预效应,探索瑞舒伐他汀抑制盐皮质激素致心肌纤维化过程的可能机制。方法 60只雄性SD大鼠行右肾切除术,术后给予1%Nacl、0.2%Kcl饮水4周并随机分为对照组(CON组),DOCA组和DOCA+ROS组。苦味酸-天狼猩红染色观察大鼠心肌间质胶原容积分数(CVF)和心肌血管周围胶原面积比(PVCA),苏木精染色观察心肌间质炎症反应程度,免疫组化法观察心肌组织单核巨噬细胞抗原(ED-1)表达,免疫印迹法检测腱糖蛋白-C表达。结果第28 d DOCA组出现明显纤维化,其CVF和PVCA指标显著高于DOCA+ROS组(P=0.02),DOCA+ROS组与CON组的CVF及PVCA差异无统计学意义(P=0.07)。与CON组相比,DOCA组、DOCA+ROS组心肌间质可见明显炎症渗出及单核巨噬细胞浸润。CON组和DOCA+ROS组腱糖蛋白-C表达差异无统计学意义(P=0.08),但均明显低于DOCA组(P=0.01)。结论盐皮质激素致心肌纤维化与心肌组织的炎症反应有关,ROS具备抑制DOCA-盐诱导的大鼠心肌纤维化作用,其机制可能与其抑制了盐皮质激素致心肌组织的炎症反应过程、减少心肌纤维化指标的表达相关。Objective To investigate whether rosuvastatin would suppress myocardial fibrosis in deoxycorticosterone - acetate （ DOCA）/sah hypertensive rats. Methods Sixty male unilateral nephrectomy SD rats were treated with 1% sodium chloride and 0.2% po- tassium chloride in the drinking water for 4 weeks and assigned to three groups： vehicle control rats （CON group）, deoxycorticosterone trea- ted group （DOCA group）, and deoxycorticosterone and rosuvastatin treated group（ DOCA ＋ ROS group）. Collagen volume fraction （CVF） was analyzed by Sirius - Red staining. Myocardial tissue inflammation was analyzed by Hematoxylin - eosin staining. Ectodermal dysplasia - 1 （El） - 1 ） was analyzed by immunohistochemistry. Expression of Tenascin - C was detected by Western blot. Results DOCA group showed severe myocardial fibrosis on 28 days, and the CVF index was that of higher than DOCA ＋ ROS group apparently （ P = 0.02）. No signifi- cant difference of CVF between the DOCA ＋ ROS group and CON group was found （P = 0.07）. Compared with CON group, different degrees of myocardial tissue inflammation and monocyte/macrophage infiltration were observed in DOCA group and DOCA ＋ ROS group. Expressions of Tenascin - C had no significant difference between CON group and DOCA ＋ ROS （ P = 0.08）, but they were lower than those in DOCA Group （P 〈 O. 01 ）. Conclusion Mineraloeorticoid - induced myocardial fibrosis is related to cardiac tissue inflammatory response. Rosuvas- tatin showed an inhibitory effect on myocardial fibrosis in the heart of DOCA/salt rats, the mechanism may be associated with the inhibition of the inflammatory response, reducing the expression of myocardial fibrosis.

关 键 词：去氧皮质酮 心肌纤维化 瑞舒伐他汀 腱糖蛋白-C 

分 类 号：R965[医药卫生—药理学] R541[医药卫生—药学]