胡黄连苷Ⅱ对大鼠肾缺血再灌注损伤的保护作用  

Protective effects and mechanism of Picroside II on renal ischemia-reperfusion in rats

作　　者：许瑞瑞[1] 张月月[1] 徐岩[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003

出　　处：《山东医药》2013年第36期7-10,I0002,共5页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(NO.ZR2011HM053)

摘　　要：目的探讨胡黄连苷Ⅱ对肾缺血再灌注(I/R)损伤的相关保护作用。方法健康雄性Wistar大鼠40只随机分为A、B、C、D、E组,A组仅暴露肾动脉不结扎,B^E组建立I/R模型,C、D、E组造模前20 min分别腹腔注射胡黄连苷Ⅱ5、10、20 mg/kg,再灌注24 h后处死动物留取血液和肾脏组织。全自动生化仪检测血清尿素氮(BUN)、肌酐(Cr)水平。硫代巴比妥酸法、比色法分别测定肾脏组织中的丙二醛(MDA)、谷胱甘肽(GSH),HE染色法观察肾脏病理变化。免疫组化法检测肾脏组织分别肿瘤坏死因子相关受体6(TRAF6)、天冬氨酸特异性半胱氨酸蛋白酶3(Caspase-3)蛋白。结果 B组与A组相比MDA、TRAF6、Caspase-3明显增高,GSH明显降低,P均<0.05;C、D、E组与B组相比MDA、TRAF6、Caspase-3明显降低,GSH明显升高,P均<0.05;D、E组各指标比较无显著性差异,与C组相比,P均<0.05。结论胡黄连苷Ⅱ对肾I/R损伤具有保护作用,与剂量大小有一定关系;其机制可能是减少氧化应激,进而增加TRAF6表达,从而减少细胞凋亡的发生。Objective To investigate the protective effects of Picroside II on renal ischemia-reperfusion （I/R） of rats. Methods Forty healthy male Wistar rats were randomly divided into five groups： sham operation group, I/R group, low-dose of Picroside II group, middle-dose of Picroside II group, high-dose of Picroside II group; and all rats were sacrificed at 24 h after reperfusion to obtain blood and kidney tissues. Serum levels of creatinine （Cr） and urea nitrogen （BUN） were determined by automatic biochemical analyzer, GSH content of renal tissues were detected by colorimetry and MDA content of renal tissues by Thiobaituricacid. The renal pathological alterations were observed by HE staining, the expression of tumor-necrosis factor receptor-associated factor 6 （TRAF6） and Caspase-3 were detected by immunohistochemistry. Results Compared with the sham operation group, the contents of MDA, TRAF6 and Caspase-3 were significantly increased, but the content of GSH was deceased in the I/R group. Compared with the I/R group, the contents of MDA, TRAF6 and Caspase-3 were significantly decreased, but the content of GSH was increased in the low-dose, middle-dose and high-dose of Picroside I1 groups, which had statistically significant differences. Moreover, no significant differences were found between the low-dose and high-dose of Picroside II groups, but with significant difference as compared with that of the low-dose of Picroside II group. Conclusions Picroside II may provide beneficial actions by reducing oxidative stress, which can reduce apoptosis by up-regulating TRAF6 expression. The protective effect of Picroside II on kidney has a dose-dependent manner.

关 键 词：肾脏损伤 缺血再灌注 胡黄连苷Ⅱ 肿瘤坏死因子相关受体6 丙二醛 谷胱甘肽 天冬氨酸特异性性半胱氨酸蛋白酶 

分 类 号：R692[医药卫生—泌尿科学]