川西獐牙菜的~1H-NMR指纹图谱研究  

Fingerprints of Swertia Mussotii by Using ~1H-NMR-based Analysis Technique

作　　者：范刚[1] 周向东[2] 罗维早[3] 罗尚华[1] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]第三军医大学医学检验系暨药学院药物化学教研室,重庆400038 [3]重庆市中药研究院,重庆400065

出　　处：《成都中医药大学学报》2013年第3期1-3,9,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家"十一五"科技支撑计划项目(2006BAI06A11-12);四川省省属高校科研创新团队建设计划项目(11TD004)

摘　　要：目的:建立川西獐牙菜药材的1H-NMR指纹图谱,为其质量评价提供新方法。方法:以氘代甲醇和重水为提取溶剂,采用1H-NMR分析技术建立指纹图谱,并利用相关系数法计算不同批次药材指纹图谱的相似度。结果:从川西獐牙菜提取物中共鉴定了25个初生和次生代谢产物,包括五环三萜类、环稀醚萜苷类、口山酮及其苷类、黄酮类、氨基酸类、糖类、脂肪酸类及有机酸类等成分。另外,10批药材指纹图谱的平均相似度为0.981。结论:1H-NMR分析方法具有整体性的特征,并且样品制备简单、分析快速、重复性好,建立的指纹图谱为川西獐牙菜药材的质量控制与评价提供了科学依据。Objective： The aims of this study were to establish the ~H-NMR fingerprints of S. mussotii, and to provide a new method for the quality evaluation of S. mu^otii. Methods： Deutcrated methanol and deuterimn oxide were used as extracting solvent, and 1H NMR-based analysis technique was applied. Moreover, correlation coefficient method was used to calculate the similarity of the IH-NMR fingerprints from different batches. Results： 25 metabolites belonging to different chemical types were detected from S. mu.~otii, including xanthones, iridoid glycosides, pentacyelic triterpenoids, flavonoids, amino acids, sugars, fatty acids, or- ganic acids, and so on. In addition, the average similarity of IH-NMR fingerprints of 10 hatches samples was 0. 981. Conclusion：Ill NMR-based analysis method had a holistic characteristic. Sample preparation and handling was simple and easy. It usually took a much shorter analysis time compared with conventional HPLC method. The results of this study can offer an important reference for the species identification and quality control of S. mu^otii or other related herb medicines.

关 键 词：川西獐牙菜 1H-NMR 指纹图谱 质量控制 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]