南方鲜食葡萄品种遗传多样性的ISSR分析  

Genetic Diversity of Table Grape Varieties in Southern China by ISSR Markers

作　　者：崔鹏[1] 李波[1] 吴月燕[2] 周伟军[1] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310058 [2]浙江万里学院生物与环境学院,浙江宁波315100

出　　处：《核农学报》2013年第9期1270-1275,共6页Journal of Nuclear Agricultural Sciences

基　　金：宁波市科技创新团队项目(2011B82019);宁波市重点委托项目(2009C10003);浙江省重中之重开放基金(KF2012005)

摘　　要：本试验利用ISSR标记研究南方45个葡萄主要栽培品种的遗传多样性和亲缘关系。从100条引物中筛选出26条带型清晰,多态性较高的引物,共扩增出310条带,多态性条带283条,平均多态性位点百分率为91%。利用UPGMA法构建聚类树状图,45个葡萄品种的遗传相似系数变化范围在0.603-0.908之间,在相似系数为0.703处将45个供试品种分为两个类群,欧亚种群和欧美杂种群。美峰与美秋的品种类型未知,二者与宇选4号的遗传相似系数分别为0.902和0.898,推测其属于欧美杂种的巨峰系列。金皇后归于欧亚种,其亲本来源有待进一步研究。Genetic diversity and genetic relationship of 45 varieties of table grape （Vitis L.） were analyzed by Inter-Simple Sequence Repeat （ISSR） markers in southern China. Twenty-six highly efficient amplification primers were selected from 100 arbitrary primers., 283 of the total 310 bands amplified showed polymorphisms, and the mean ratio of polymorphism was 91%. The genetic similarity coefficient among the 45 samples floated from 0.603 to 0.908. The clustering dendrogram was constructed by UPGMA method. At the point of 0.703, all the samples were classified into two groups： V. vinifera L. and hybrid of V. vinifera L.×V. labrusca L.. The varietal typesof Meifeng and Meiqiu were unknown. According to the clustering analysis, the similarity coefficient between Meifeng, Meiqiu and Yuxuan 4 was 0.902 and 0.898 respectively, which suggested that the two varieties belong to pedigree of Kyoho grapevine series, hybrids of V. vinifera L.×V. labrusca L. Golden Queen might belong to V. vinifera L., and a further study of its parental origin is needed.

关 键 词：葡萄 南方 ISSR 聚类分析 遗传多样性 

分 类 号：S663.1[农业科学—果树学]