共轭亚油酸同分异构体对肥胖Zucker大鼠肾脏中的类二十烷酸的影响  

Effect of Conjugated Linoleic Acid Isomeride on Kidney Eicosanoid of fa/fa Zucker Rats

作　　者：石红[1] 洪晶安[2] 

机构地区：[1]玉溪市人民医院临床营养科,云南玉溪653100 [2]云南省第一人民医院临床营养科,昆明650032

出　　处：《中国食物与营养》2013年第9期73-77,共5页Food and Nutrition in China

摘　　要：目的:观察共轭亚油酸(CLA)的两种同分异构体对17w的肥胖Zucker大鼠的肾组织中的类二十烷酸水平的影响,并探测与这些类二十烷酸合成有关的酶的蛋白表达。方法:17w的雄性Zucker大鼠随机分为对照组[瘦鼠对照组(lean control)和胖鼠对照组(fa/fa control),n=7]和治疗组[随机抽取的肥胖鼠(fa/fa 9,11CLA和fa/fa 10,12 CLA),n=7],分别接受无CLA的对照饮食或加入0.4%(w/w)CLA的饮食。8w后,用高效液相质谱仪(HPLC/MS/MS)检测多种类二十烷酸水平、用免疫印迹法(Westernblotting)检测与这些类二十烷酸合成有关的酶的蛋白表达。结果:t10,c12 CLA增加了环氧合酶COX1的蛋白表达(P<0.05)、升高了内源性的TXB2水平(P<0.05)。COX1的升高与血柱素B2(TXB2)升高呈正相关。t10,c12 CLA降低了外源性的13-HODE和总的HODEs水平(P<0.001)以及外源性的15-HETE(P<0.01)、5-HETE(P<0.01)、8-HETE(P<0.05)和总外源性HETEs(P<0.05)。结论:t10,c12 CLA增加了COX1的蛋白表达,继而升高了TXB2水平,加重了肾组织损伤。t10,c12 CLA降低了外源性的15-HETE、5-HETE和8-HETE水平,由于同时降低了脂氧素,可能参与了对肾脏的损伤。[Objective] To investigate the effect of CLA isomers on kidney eicosanoid of 17 week old male Zucker rats and detect the protein levels of enzymes involved in the synthesis of these eicosanoids. [Method] 17 week old male Zucker rats were randomly assigned into control groups fed with control diet without CLA and treatment groups fed with 0. 4% （w/w） CLA isomers respectively. After 8 weeks, HPLC/MS/MS was used to exam the eicosanoid levels. Westernblotting was used to detect the protein levels of enzymes involved in the syn- thesis of these eicosanoids. [Result] t10, c12 CLA increased the protein levels of COX1 （P 〈 0. 05） and endogenous levels of TXB2 （P 〈0. 05） . The increase of COX1 protein level was positively correlated to endogenous levels of TXB2. t10, c12 CLA decreased the in vitro production of 13-HODE and total HODEs （P 〈 0. 001 ） and 15-HETE （P 〈 0. 01 ）, 5-HETE （ P 〈 0. 01 ）, 8-HETE （ P 〈 0. 05 ） and total HETEs （P 〈0. 05）. [Conclusion] tl0, el2 CLA worsened the histological damage of kidney through the inerease of COX1 protein levels and TXBzlevels caused by COX1. The reduction of the in vitro production of several HETEs by t10, e12 CLA contributed to the damage of kidney because of the decrease of lipoxins.

关 键 词：共轭亚油酸同分异构体 类二十烷酸 Zucker大鼠 

分 类 号：R589.2[医药卫生—内分泌]