107份人血清布鲁氏菌病两种检测方法比较  

Comparison of two methods for brucellosis testing in 107 human sera

作　　者：邵宏业[1] 张帅清[1] 

机构地区：[1]河南省三门峡市疾病预防控制中心,河南三门峡472000

出　　处：《中国卫生检验杂志》2013年第10期2292-2294,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的探讨虎红平板凝集反应(RBRT)与试管凝集反应(SAT)在人布鲁氏菌病(简称布病)检测中的应用及效果。方法同时采用WS 269-2007《布鲁氏菌病诊断标准》的RBRT、SAT方法对107份人血清进行布病诊断检测,分析检测结果。结果 SAT"布病及疑似布病"的检出率与RBRT"≥+"的检出率差异有统计学意义(χ2=29.789,P<0.005)。RBRT与SAT相比:敏感性为91.42%(64/70)、特异性为56.76%(21/37)、符合率为79.4%(85/107);两种检测结果呈一定的正相关性。结论大面积布病检测时,宜先RBRT筛查再SAT确诊。非大面积布病检测时,不必先用RBRT筛查,直接用SAT检测。且可依RBRT结果大致推断SAT结果,这对布病暴发的早期识别,紧急预防和治疗等意义重大。Objective To investigate the application and effect of RBRT and SAT in the diagnosis of human brucel- losis. Methods One hundred and seven human sera were tested by RBRT and SAT according to WS 269 -2007 ＂Brucellosis diagnostic criteria＂ respectively. Then the brucellosis diagnosis results by the two methods were ana- lyzed. Results The positive rate had significant difference between the ＂suspected brucellosis and brucellosis ＂ of SAT and ＂ ≥ ＋ ＂ of RBRT （X^2 =29. 789, P 〈0. 005）. The sensitivity was 91.42% （64/70）, the specificity was 56.76% （21/37） , and the coincidence rate was 79.4% for RBRT comparing with SAT. The two kinds of test re- sults showed positive correlation in a certain extent. Conclusion RBRT screening and SAT confirmation is better in large -scale brucellosis detection, while only direct SAT detection is enough in non large -scale brucellosis de- tection. In addition, SAT results deduced from RBRT results is significant for early recognition, emergency preven- tion and treatment of brucellosis.

关 键 词：人血清 布鲁氏菌病 虎红平板凝集反应 试管凝集反应 比较 

分 类 号：R446.11[医药卫生—诊断学]