瑞舒伐他汀对大鼠急性心肌梗死后梗死组织miR-126的表达及血管再生的影响  

Effects of rosuvastatin on angiogenesis and expression of miR-126 after acute myocardial infarction in rats

作　　者：孙涛[1] 万大国[1] 宋恒良[1] 孙慎杰[1] 

机构地区：[1]郑州大学第二附属医院心内科,郑州450014

出　　处：《郑州大学学报（医学版）》2013年第5期640-643,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:探讨瑞舒伐他汀能否上调大鼠急性心肌梗死后梗死组织miR-126的表达以及其与血管再生的关系。方法:54只大鼠建立急性心肌梗死模型后随机分为模型组27只和瑞舒伐他汀组27只。次日起瑞舒伐他汀组给予瑞舒伐他汀10 mg/(kg·d)连续灌胃6周,模型组给予等量助溶溶剂连续灌胃6周。处死动物,取梗死组织,采用免疫组化SP法检测心肌组织微血管密度(MVD),采用实时荧光定量PCR法检测miR-126的表达水平。结果:与模型组相比,瑞舒伐他汀组梗死组织MVD增大,miR-126的表达水平升高(t=2.386和3.264,P<0.05);瑞舒伐他汀组梗死组织MVD和miR-126的表达水平呈正相关(r=0.720,P=0.026)。结论:瑞舒伐他汀可能通过上调miR-126的表达促进心肌梗死大鼠梗死心肌组织的血管再生。Aim：To investigate whether rosuvastatin could up-regulate the expression of miR-126 after acute myocardi-al infarction in rats and study its relationship with angiogenesis .Methods：A total of 54 rats were established acute myocar-dial infarction model and randomly allocated into the model group and the rosuvastatin group with 27 in each group .Then the rats in the rosuvastatin group were given rosuvastatin at 10 mg/（kg· d） by gavage for 6 weeks, and those in the model group were given equivalent solvent .After 6 weeks,the rats were executed and the myocardial infarction samples were pre-pared .The myocardial microvessel density was detected by immunohistochemical staining , and the expression level of miR-126 was determined by real time PCR .Results：Compared with the model group , the microvessel density and the expres-sion level of miR-126 in rosuvastatin group obviously elevated （t=2.386 and 3.264,P 〈0.05）; the 2 parameters were positively related in myocardial infarction tissue of rosuvastatin group （r=0.720, P=0.026）.Conclusion： Rosuvastatin may promote angiogenesis in rat infarction myocardium by upregulating the expression of miR -126.

关 键 词：瑞舒伐他汀 MIR-126 血管再生 急性心肌梗死 大鼠 

分 类 号：R542.2[医药卫生—心血管疾病]