拟南芥冷害胁迫下基因表达谱的统合分析  

Meta-analysis of Gene Expression Profile Under Cold Stress of Arabidopsis

作　　者：徐兆华[1] 吴为人[1,2] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310058 [2]福建农林大学作物科学学院,福建福州350002

出　　处：《华南农业大学学报》2013年第4期511-516,共6页Journal of South China Agricultural University

基　　金：973项目(2004CB117306)

摘　　要：对4套不同来源的拟南芥冷胁迫芯片试验数据进行了统合分析,并与各试验单独分析的结果进行了比较.结果表明,那些在2个或2个以上试验中被同时检测到的"较可靠的"差异表达基因(Differentially expressed gene,DEG)大多数都能被统合分析检测出来,而那些只在1个试验中被检测到的"较不可靠的"DEG则大约只有1/4被统合分析检测到,说明统合分析适合于检测"较可靠的"DEG.在统合分析检测到的DEG中,约有9%是未被单独分析检测到的,其中包含6个与冷害胁迫直接相关的基因,说明统合分析还可以检测到被单独分析忽略的DEG.基因本体和启动子基序分析显示,统合分析检测到的DEG确实与冷胁迫有着密切的关系.Meta-analysis of microarray data from four published cold stress experiments in Arabidopsis thaliana were performed to detect differentially expressed genes （ DEGs） .The results were compared with those obtained by the separate analyses of the individual experiments .The results showed that most of those “more reliable” genes, which were detected in two or more experiments , were detectable in the meta-analysis, whereas merely a quarter of those “less reliable” genes, which were detected only in a single experiment , were detectable in the meta-analysis .This suggested that meta-analysis was suitable for detecting “more reliable” DEGs.It was noticeable that 9%of the genes detected by the meta-analy-sis, among which 6 genes were directly related to the response to cold stress , were not detected by the separate analyses , indicating that meta-analysis could detect some DEGs neglected by separate analyses . Gene ontology （ GO） and promoter motif analyses suggested that the DEGs detected by the meta-analysis were indeed closely related to cold response .

关 键 词：冷胁迫 微阵列 差异表达基因 统合分析 

分 类 号：S511[农业科学—作物学] S502