PTEN、MGMT和DNA-PKcs在男性乳房发育症乳腺中的表达及其生物学意义  

Expression of PTEN,MGMT and DNA-PKcs in gynecomastia and their biological significance

作　　者：朱凌冬[1] 远里美 马丽霞[1] 于旸[1] 孙立泉[1] 梁向荣[1] 蔡景龙 

机构地区：[1]山东大学齐鲁儿童医院外二科,济南250022 [2]济南市第五人民医院妇产科,济南250022 [3]中国医学科学院整形外科医院瘢痕治疗中心,北京100144

出　　处：《诊断病理学杂志》2013年第9期571-575,共5页Chinese Journal of Diagnostic Pathology

摘　　要：目的观察PTEN、MGMT和DNA-PKcs在男性乳房发育症(GM)乳腺中的表达及其意义。方法采用免疫组化SP法检测68例GM乳腺(实验组)与24例正常男性乳腺(对照组)中PTEN、MGMT和DNA-PKcs的表达状况,并将实验组按组织学类型(硬化型、过渡型、旺炽型)分为3组分别研究。结果 PTEN、MGMT和DNA-PKcs在GM乳腺与正常男性乳腺中均有表达。GM乳腺中的3项表达水平较正常男性乳腺显著降低(P<0.01),硬化型、过渡型、旺炽型中3项表达水平也依次降低。PTEN、MGMT和DNA-PKcs的表达两两之间比较无显著相关性(P>0.05)。结论 GM乳腺组织中PTEN、MGMT和DNA-PKcs表达的异常可能与GM的发生有关。Objective To investigate the expression and significance of PTEN, MGMT and DNA-PKcs protein in gynecomastia. Methods Immunohistochemical SP method was used to detect the expression of PTEN, MGMT and DNA- PKcs protein in 68 cases of gynecomastia （ experiment group ） and 24 cases of mammary gland of normal man （ control group）. The selected examples were divided into three different histological types （constrictive type, transitional type, and prosperous and intense type）. Results In both gynecomastia and mammary gland of normal man, the PTEN, MGMT and DNA-PKcs protein were expressed. The expression level of PTEN, MGMT and DNA-PKcs protein in gynecomastia was significantly lower than in mammary gland of normal man tissues （P 〈 0. 01 ）. The expression difference of PTEN, MGMT and DNA-PKcs protein in three different histological types was statistically significant （P 〈 0. 05）, and the expression level of them degraded in constrictive type, transitional type, and prosperous and intense type by turns. Conclusion This study suggests that the abnormal expression of PTEN, MGMT and DNA-PKcs in gynecomastia probably plays an important role in the development of gynecomastia.

关 键 词：男性乳房发育症 张力蛋白同源物基因 6-氧-甲基鸟嘌呤-DNA甲基转移酶 DNA依赖蛋白激酶 免疫组化 

分 类 号：R655.802[医药卫生—外科学]