Ag^+与Hg^(2+)对G-四链体DNA结构的破坏及其对构筑DNA逻辑门的应用(英文)  

Disruption of G-Quadruplex DNA by Ag^+ and Hg^(2+) and Its Application to DNA Logic Gate

作　　者：朱莉娜[1] 王海仙[1] 孙润丰[2] 李平[3] 孔德明[3] 李孝增[1] 

机构地区：[1]天津大学化学系,天津300072 [2]青海民族学院外语系,西宁810007 [3]南开大学教育部功能高分子材料重点实验室,天津300071

出　　处：《无机化学学报》2013年第10期2215-2224,共10页Chinese Journal of Inorganic Chemistry

基　　金：国家自然科学基金(No.20801040,21175072)资助项目

摘　　要：本工作利用圆二色光谱研究了Ag+与Hg2+对4种代表性G-四链体DNA结构的破坏作用。结果表明Ag+可能通过与碱基G螯合从而破坏G-四链体结构;Hg2+能通过形成T-Hg2+-T碱基对,及其他方式破坏G-四链体结构。含巯基(-SH)的半胱氨酸与Ag+与Hg2+可以发生较强的配位作用,从而使被Ag+与Hg2+破坏后的G-四链体DNA结构得以回复。基于此,一个新颖的Ag+/Hg2+-半胱氨酸-DNA逻辑门得以构筑。The G-quadruplex-disrupting abilities of Ag＋ and Hg2＋ ions were investigated by utilizing the metal ions-mediated circular dichroism （CD） spectrum changes of four representative G-quadruplexes. The resuhs show that Ag＋ ion can disrupt G-quadruplexes by chelating to G bases and thus can be used as a general G- quadruplex-disrupting agent. The results also suggest that Hg2＋ ion may disrupt G-quadruplexes by formation of T-Hg〉-T base pairs as well as other species. The G-quadruplexes disrupted by Ag＋ and Hg2＋ can be recovered by cysteine （Cys）, a thiol-containing amino acid, due to the tight binding interactions between Cys and the metal ions. Thus, the system by using Ag＋ （or Hg2＋） and Cys as two inputs and CD signal as output can behave as a DNA IMPLICATION logic gate.

关 键 词：G-四链体 DNA逻辑门 银离子 汞离子 半胱氨酸 圆二色光谱 

分 类 号：O614.122[理学—无机化学] O614.243[理学—化学]