取食不同寄主植物小菜蛾对氯虫苯甲酰胺敏感性及体内解毒酶活性的变化  

Changes in susceptibility to chlorantraniliprole and detoxification enzymes of Plutella xylostella feeding on different host plants

作　　者：尹飞[1] 陈焕瑜[1] 李振宇[1] 林庆胜[1] 胡珍娣[1] 张德雍[1] 冯夏[1] 

机构地区：[1]广东省农业科学院植物保护研究所广东省植物保护新技术重点实验室,广州510640

出　　处：《应用昆虫学报》2013年第5期1335-1340,共6页Chinese Journal of Applied Entomology

基　　金：公益性行业(农业)科研专项(201103021);国家支撑项目(2012BAD19B0);浙江省省部共建国家重点实验室培育基地开放基金项目(2010DS700124-KF1207);广州市农业攻关(GZCQC1202FG04002);广东省自然科学基金项目(10451064001006248和10151064001000025);广东省农业科学院院长基金(201012);促进科技服务业发展计划(2011B040100005)

摘　　要：为探明寄主植物影响小菜蛾Plutella xylostella L.抗药性的内在因素,本研究以小菜蛾嗜好性不同的菜心(Brassica parachinensis)、芥菜(B.juncea)、白菜(B.chinensis)和芥蓝(B.alboglabra)4种寄主植物为材料,利用生物测定和解毒酶活力测定的方法,研究了取食不同寄主植物的小菜蛾幼虫对氯虫苯甲酰胺敏感性及体内解毒酶活力变化情况。结果表明,4种寄主植物饲养小菜蛾5代后,其敏感性分别为菜心>芥菜>白菜>芥蓝,且其体内活性酶谷胱甘肽S-转移酶(GSTs)、羧酸酯酶(CarE)比活力差异显著(P<0.05),活性的强弱与其敏感性相一致。以取食芥蓝的小菜蛾体内2种解毒酶活性最强。说明寄主植物能影响小菜蛾药剂敏感性及解毒酶活性。试验结果对小菜蛾的综合防控及抗氯虫苯甲酰胺机理研究具有重要的参考价值。In order to ascertain the internal factors that host plants infect Plutella xylostella （DBM：Diamondback moth） resistance, the susceptibility to chlorantraniliprole and enzymic activity of DBM larva were tested with bioassay and biochemical methods. The DBM larva were fed with 4 preferred species host plants which were Brassica parachinensis, B. juncea, B. chinensis and B. alboglabra. The results showed that the most susceptible DBM larva was those fed with B. alboglabra, followed by B. juncea, B. chinensis and B. alboglabra after they were raised 5 generations, respectively. Glutathione-S-transferase （GSTs） and carboxylesterase （CarE ） indicated significant difference at 0. 05 level. The most activity of GSTs and CarE was showed in the DBM fed with B. alboglabra. All results indicated that feeding on different host plants could affect the pesticide susceptibility and detoxification enzyme activity of DBM. The results showed important reference value to IPM and resistance mechanism of DBM.

关 键 词：小菜蛾 寄主植物 氯虫苯甲酰胺 解毒酶活性 敏感性 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]