蜜蜂羽化温度与卷翅病毒动态复制相关性研究  

The correlation between bee (Apis mellifera) emergence temperature and replication dynamics of the deformed wing virus

作　　者：张炫[1,2] 陈彦平[2] 周丹银[1] 和绍禹[1] 

机构地区：[1]云南农业大学东方蜜蜂研究所,昆明650201 [2]美国农业部东部蜜蜂研究室,马里兰州20705

出　　处：《应用昆虫学报》2013年第5期1341-1347,共7页Chinese Journal of Applied Entomology

基　　金：现代农业(蜜蜂)产业技术体系建设专项基金(CARS-45-kxj14);美国农业部CAP基金(2010-85118-05718)

摘　　要：病毒复制动态变化对于病毒病的研究有着重要意义。选用蜜蜂卷翅病毒(deformed wing virus,以下简称DWV)作为研究模型,检测DWV隐性感染蜂群在不同温度条件下羽化工蜂体内的病毒浓度,以了解不适温度压力这一非生物因子对蜜蜂羽化率及蜜蜂体内DWV病毒复制动态的影响。实验结果显示不适羽化温度不仅会明显降低蜜蜂羽化成功率,而且新羽化的带毒蜜蜂体内DWV病毒拷贝量增加极显著。部分解释了冬季和早春低温环境下因病毒病暴发造成人工饲养蜂群非正常损失的原因。Understanding the replication dynamics of viruses is vital to the study of disease pathogenesis. The dynamics of the deformed wing virus （DWV） in honeybee hosts was investigated. Pupae from a colony with no apparent DWV infection were bred at different temperatures. Newly emerged worker bees were collected and virus titers calculated. We found that unsuitable emergence temperatures cause a sharp decline in adult emergence from pupae. Simultaneously, DWV titers significantly increased in the adults that did emerge. These results partly explain abnormal colony losses during periods of low temperature in winter and early spring. Our laboratory studies support the correlation between abiotic stress factors and the dynamics of virus replication in honey bees.

关 键 词：西方蜜蜂 卷翅病毒 羽化温度 病毒浓度 

分 类 号：S895[农业科学—特种经济动物饲养]