一氧化氮对星形胶质细胞轴突生长因子-1表达及迁移的影响  

Effects of nitric oxide on netrin-1 expression and migration of astrocytes

作　　者：施月[1] 张晔[1] 邵杰[1] 姚扬明[1] 夏春林[1] 

机构地区：[1]苏州大学医学部博习临床研究所暨细胞神经生物研究室,苏州215123

出　　处：《解剖学报》2013年第5期635-640,共6页Acta Anatomica Sinica

摘　　要：目的探讨一氧化氮(NO)对星形胶质细胞中轴突生长因子-1(netrin-1)的表达变化以及对细胞迁移的影响。方法采用硝普钠(SNP)作为NO供体处理星形胶质细胞,通过振荡培养和差速贴壁法分离纯化新生SD大鼠星形胶质细胞,接种至培养板,分为实验组和对照组,每组6个样本,实验组用50μmol/L SNP处理星形胶质细胞,通过划痕法观察细胞的迁移,并采用Western blotting和免疫细胞化学方法分别检测处理前后的星形胶质细胞中netrin-1蛋白的表达变化。结果 SNP处理后,实验组的星形胶质细胞与对照组相比,划痕区细胞明显增多,星形胶质细胞netrin-1表达随着时间的推移出现先升高后降低趋势,于48 h达到峰值(P<0.01)。结论 SNP使星形胶质细胞netrin-1表达水平发生变化和影响星形胶质细胞迁移,提示netrin-1可能通过NO的调控而影响星形胶质细胞迁移。Objective To investigate effects of NO on netrin-1 expression and migration of astrocytes. Methods Astrocytes were treated with a certain concentration of NO donor sodium nitroprusside （SNP） in vitro.The astrocyte migration was observed and the expression of netrin-1 at the different times after treatment was detected. Purified cultures of astrocytes were prepared from neonatal rats, based on the differential properties of developmental time-course and cellular adhesions. Astrocytes were inoculated to culture plates and were divided into experimental and control groups （n=6）.The experimental groups were treated with 50μmol/L NO donor SNP. Cell migration was observed by scratching. The expression of netrin-1 after treatment was examined by Western blotting and immunocytochemistry. Results The speed of astrocyte migration increased after SNP treatment. The expression of netrin-1 in the protein level began to increase at 6 hours and reached the peak at 48 hours, compared with the control group （P〈0.01）, and then declined. Conclusion SNP may increase the expression of netrin-1 protein and promote astrocytes migration, indicating that netrin-1 promoting astrocytes migration maybe controled by NO.

关 键 词：星形胶质细胞 轴突生长因子-1 一氧化氮 细胞迁移 免疫印迹法 大鼠 

分 类 号：Q256[生物学—细胞生物学]