电针对青春期前后大鼠下丘脑NPY表达的影响  

Effects of electro-acupuncture on expression of neuropeptide Y in hypothalamus of rats during pubertal development

作　　者：卢静[1] 丁贵鹏 王雪松 仲远明[1] 崔毓桂 张朝晖[1] 

机构地区：[1]南京医科大学第一附属医院针灸科,江苏南京210029 [2]南京医科大学第一附属医院生殖医学中心实验室,江苏南京210036

出　　处：《南京医科大学学报（自然科学版）》2013年第9期1214-1219,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省135医学重点人才项目(RC2002076)

摘　　要：目的:电针(EA)对青春发育早期正常大鼠下丘脑促性腺激素释放激素(GnRH)具有显著降调节作用。为揭示该效应的内在机制,本研究观察了电针后不同发育阶段大鼠下丘脑神经肽Y(NPY)表达的作用,以确定NPY是否是抑制GnRH释放调控的重要因素之一。方法:在4个不同发育阶段,分别给予电针穴位组和电针非穴位组大鼠以低频电针(3 Hz)持续治疗10 d,每天20 min。10 d后,测定下丘脑NPY的表达。结果:电针穴位组青春发育早期大鼠下丘脑NPY的表达显著低于空白组(P<0.05);与大鼠下丘脑GnRH表达的变化相比,电针穴位组青春发育早期大鼠下丘脑NPY表达与GnRH表达的变化呈同向波动趋势;而NPY表达的下降不影响大鼠体重的增加。结论:低频电针可有效下调青春发育早期正常大鼠下丘脑NPY的表达,推测NPY不是大鼠青春发育早期下丘脑GnRH释放调控的关键因素。Objective：To confirm if neuropeptide Y（NPY） is one of the most important factors in regulating GnRH release. Methods：In this study,we investigated the change of NPY expression in hypothalamus of common rats treated with the repeated EA at different developmental stages. Low frequency EA（3 Hz） was performed at acupoints（treatment groups） or non-acupoints （control groups） for 20 min daily for 10 days in Sprague-Dawley （SD） rats at four developmental stages,which were juvenile stage,early puberty stage,later puberty stage and adult stage. NPY expression in the hypothalamus was determined after 10-day treatment. Results：The results showed that NPY expression in the early pubertal group（EPG） was significantly depressed after repeated EA（P 〈 0.05）. Compared with the results of GnRH expression and body weights,the change of NPY expression was similar with the fluctuation of GnRH expression after EA and the increase of body weights of rats was not influenced by the depression of NPY expression after EA during early puberty. Conclusion：The results demonstrated that repeated low frequency EA was an effective method on down-regulating NPY expression in the hypothalamus and we inferred that NPY was not the key role on regulating GnRH release in hypothalamus of common rats at early puberty stage.

关 键 词：电针 下丘脑-垂体 神经肽Y(NPY) 大鼠 

分 类 号：R338.27[医药卫生—人体生理学]