雪灵芝水提物对淋巴细胞增殖及Th1型细胞因子表达的影响  

Effects of Arenaria kansuensis aqueous extract on lymphocytes proliferation and Th1 cytokine production

作　　者：王刚[1] 王海燕[1] 张华[1] 贺菊香[1] 吉利宾[2] 杨歆睿[2] 

机构地区：[1]青海大学医学院病理学教研室,西宁810001 [2]青海大学医学院,西宁810001

出　　处：《中国免疫学杂志》2013年第9期905-909,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81060369);青海省应用基础研究项目(2010-Z-722)资助

摘　　要：目的：探讨雪灵芝水溶性提取物对淋巴细胞增殖及其Th1型细胞因子表达的影响。方法：体外培养的小鼠脾淋巴细胞及人外周血单个核细胞（PBMC）经不同浓度雪灵芝水提物处理。采用MTT法检测小鼠脾淋巴细胞增殖水平;应用ELISA双抗体夹心法检测小鼠脾淋巴细胞培养上清中IL-2和IFN-γ水平;RT-PCR与实时定量PCR检测人PBMC中NF-кB p65、IL-2及IFN-γ的mRNA表达。结果：雪灵芝水提物在0.025~0.2 mg/ml浓度范围,对小鼠脾淋巴细胞增殖具有浓度依赖性的促进作用;0.05 mg/ml和0.1 mg/ml雪灵芝处理组IL-2水平高于对照组（P〈0.05）,0.4 mg/ml雪灵芝处理组IFN-γ水平高于对照组（P〈0.05）;相对于对照组,0.2 mg/ml雪灵芝处理组人PBMC的NF-кB p65、IL-2及IFN-γmRNA相对表达系数分别为204.76、4.31和40.46。结论：雪灵芝水提物对小鼠脾淋巴细胞增殖以及小鼠脾淋巴细胞、人PBMC中Th1型细胞因子的表达具有促进作用。Objective：To investigate the effects of Arenaria kansuensis aqueous extract（AKAE） on lymphocytes proliferation and Th1 cytokine expression.Methods：Mice spleen lymphocytes and human peripheral blood monouclear cells（PBMC） were treated with AKAE at various concentration in vitro;the proliferation level of mice spleen lymphocytes was observed by MTT assay;the level of IL-2 and IFN-γ produced by mice spleen lymphocytes were tested by enzyme-linked immunosorbent assay（ELISA）;RT-PCR and realtime PCR were used to detect the mRNA expression of NF-кB p65,IL-2 and IFN-γ in human PBMC.Results：The proliferation of mice spleen lymphocytes was dose-dependently enhanceed by AKAE treatment at concentration of 0.025 ~ 0.2 mg/ml;in culture supernatant of mice spleen lymphocytes,the level of IL-2 was increased after AKAE treatment at dose of 0.05 mg/ml and 0.1 mg/ml（P0.05）,and the level of IFN-γ was increased after AKAE treatment at dose of 0.4 mg/ml（P0.05）;compared with control group,the relative expression of NF-кB p65,IL-2 and IFN-γ mRNA in human PBMC treated by 0.2 mg/ml AKAE were 204.76,4.31 and 40.46 respectively.Conclusion：AKAE could enhance the proliferation of mice spleen lymphocytes and promote the Th1 cytokine expression of mice spleen lymphocytes and homo PBMC.

关 键 词：雪灵芝 淋巴细胞 细胞增殖 TH1型细胞因子 

分 类 号：R392.12[医药卫生—免疫学]