氯化甲基汞对仔鼠小脑PKCα mRNA表达的影响  

Effects of Methylmercury chloride exposure on PKCα mRNA expression in rat brain during development

作　　者：郭杰[1] 张宏宇[2] 张雯艳[3] 李志超[4] 王铁君[1] 邢程[5] 

机构地区：[1]吉林大学第二医院放疗科,长春130041 [2]中国人民解放军208医院461临床部,长春130061 [3]吉林省妇幼保健院,长春130061 [4]吉林大学公共卫生学院,长春130021 [5]吉林省卫生厅,长春130051

出　　处：《中国免疫学杂志》2013年第9期923-925,共3页Chinese Journal of Immunology

基　　金：吉林省卫生厅科研基金资助项目(3D5097923427);吉林大学基本科研业务费专项基金资助项目(421030140427);吉林大学青年教师基金资助项目(419070210048)

摘　　要：目的:观察氯化甲基汞对仔鼠小脑中PKCαmRNA表达的影响。方法:建立氯化甲基汞致仔鼠小脑发育损伤大鼠实验模型。采用核酸原位杂交方法检测α型蛋白激酶C(PCKα)mRNA表达。结果:和对照组相比,各实验组仔鼠在出生后3天至出生后30天,小脑蒲肯野细胞PKCαmRNA表达均明显增高。小脑蒲肯野细胞PKCαmRNA表达随出生后时间的延长,其表达量逐渐增多。并且氯化甲基汞浓度与PKCαmRNA表达量呈正比,随氯化甲基汞浓度增高,小脑蒲肯野细胞PKCαmRNA表达明显增多。结论:氯化甲基汞以剂量依赖方式和时间依赖方式促进仔鼠小脑蒲肯野细胞PKCαmRNA表达。氯化甲基汞可通过上调神经元中丝氨酸/苏氨酸蛋白激酶促进小脑神经元凋亡进而损伤中枢神经系统。Objective：To investigate the effect of Methylmercury chloride exposure on PKCα mRNA expression in rat brain during development.Methods：Establishment of developmental brain injured model of rats by chronic MMC exposure.In Situ Hybridization analysis were performed to detect the expression of PKC isozyme.Results：PKCα was expressed at low levels at birth but increases significantly during postnatal development.With the increasing brain Hg2＋level,expression of PKCα in rat＇s brain from experimental groups was significantly higher than that of control group.Conclusion：Methylmercury chloride exposure can affect PKCα mRNA expression in rat brain during development,which may results in the increasing of cerebellum neuron apoptosis mediated by serinethreonine kinase pathway.

关 键 词：氯化甲基汞 脑发育 PKCαmRNA 

分 类 号：R392[医药卫生—免疫学]