不明原因复发性流产与HLA-DQ相关性的初步研究  

Study on the correlation of HLA-DQ alleles and unexplained recurrent miscar riage

作　　者：吴彤华 朱元昌 尹彪 李观贵 陈文娜 陈春媚 曾勇 梁佩燕 

机构地区：[1]深圳中山泌尿外科医院生殖医学中心深圳中山生殖与遗传研究所深圳市围着床期生殖免疫重点实验室,深圳518000

出　　处：《中国免疫学杂志》2013年第9期955-959,共5页Chinese Journal of Immunology

基　　金：广东省医学科学技术研究基金(No.B2012331)资助

摘　　要：目的:探讨夫妇双方人类白细胞抗原(Human leukocyte antigen,HLA)-DQ等位基因多态性与不明原因复发性流产之间的关系。方法:对25例不明原因复发性流产夫妇和16例正常妊娠的夫妇,采用序列特异性引物PCR和基因测序分型的方法分别检测夫妇双方HLA-DQA1和HLA-DQB1基因型,并对双方HLA-DQ的基因频率和相容性进行统计学分析。结果:女方不明原因复发性流产患者中HLA-DQA1和HLA-DQB1等位基因频率与对照组相比无统计学差异(P>0.05);在男方患者中,HLA-DQB1*03:01频率显著升高(χ2=4.02,OR=3.29,P=0.045),HLA-DQB1*05:02频率显著降低(χ2=6.38,OR=0.15,P=0.025)。两组夫妇间HLA-DQ位点的匹配个数之间无统计学差异(P>0.05)。结论:男方HLA-DQB1基因位点在不明原因复发性流产发生中起重要作用,HLA-DQB1*03∶01可能是易感基因,而HLA-DQB1*05:02可能是保护性基因。Objective：To investigate the correlation of human leukocyte antigen（HLA）-DQ allele polymorphism and unexplained recurrent miscarriage（uRM）.Methods：HLA-DQA1 and HLA-DQB1 genotyping was carried out in 25 couples with uRM（uRM group） and 16 fertile couples（control group）,using polymerase chain reaction sequence specific primer（PCR-SSP） and sequence based typing（SBT） methods.The frequency and sharing of HLA-DQ alleles were analyzed by SPSS 16.0 software.Results：In females,the distribution of HLA-DQA1 and DQB1 alleles of uRM group were similar to those of control group（P0.05）.But in males,the frequency of HLA-DQB1 ＊ 03：01 allele was significantly increased（χ2= 4.02,OR = 3.29,P = 0.045） in uRM group,whereas the frequency of HLA-DQB1＊ 05：02 allele was significantly decreased（χ2= 6.38,OR = 0.15,P = 0.025）.Furthermore,the HLA-DQ sharing between the two groups was no significant difference.Conclusion：HLA-DQB1 alleles might be partly response for the pathogenesis of uRM,especially for men.DQB1＊ 03：01 allele may be a susceptible gene,while DQB1＊ 05：02 allele may be a protective gene for uRM.

关 键 词：人类白细胞抗原 不明原因复发性流产 多态性 基因分型 

分 类 号：R392.2[医药卫生—免疫学]