贯叶金丝桃提取物对抑郁模型小鼠行为及海马神经元损伤影响的研究  

作　　者：马晓华[1] 王一奇[2] 毛水龙[1] 

机构地区：[1]浙江中医药大学基础医学院机能研究所,310053 [2]浙江中医药大学药学院,310053

出　　处：《浙江临床医学》2013年第10期1441-1444,共4页Zhejiang Clinical Medical Journal

基　　金：浙江省中医药科技计划项目（2008CB003）;浙江省教育厅科研项目（Y201017372）;浙江中医药大学校级科研项目2007年校级课题

摘　　要：目的探究贯叶金丝桃提取物（hypericumperforatumextracts，HPE）对抑郁模型小鼠行为、海马神经元损伤的影响。方法用小鼠尾悬挂实验和强迫游泳实验对HPE的抗抑郁作用进行研究；将50只昆明种小鼠分为空白对照组，氟西汀组，HPE高（1．2g／kg）、中（0．60g／kg）、低剂量（0．20g／kg）组，采用长期不可预见性中等强度应激造成小鼠慢性应激抑郁模型，Nissl染色法观察海马CAl，CA3区神经元形态及锥体细胞数目。结果与空白对照组比较，HPE能显著缩短强迫游泳小鼠及尾悬吊小鼠的不动时间，并呈一定的剂量依赖性；与空白对照组比较，氟西汀组小鼠海马CA3区锥体细胞数量明显减少（P〈0101），HPE高、中、低剂量组能明显增加海马CA3区锥体细胞数量。结论贯叶金丝桃提取物能改善小鼠的抑郁样行为，保护抑郁模型小鼠海马神经元损伤。Objective To observe the effects of hypericum perforat^n extracts （ HPE ） on behavior, injury of hippocampal neurons in the depression mice model. Method Effects of HPE on behavior in depression model mice were observed by using the mouse tail suspension test and forced-swimming test models of depression; fifty Kunming mice were randomly divided into 5 groups, the control group, the model group and three HPE groups （the doses were 1.20g/kg, 0.60g/ kg, 0.20g/kg respectively ） , The model was established by separation and chronic unpredictable mild stimulation, Cone cell and configuration of neuron in CA1, CA3 region of hippocampus were observed by Nissl. Result Comparing with the mice of the model group, immobility time of forced- swimming mice or tail suspension stress mice was significantly shortened by EHP in a dosedependent manner; comparing with the mice of control, numbers of cone cell in CA3 region of hippocampus were decreased obviously （P〈0101） in the model group, HPE （1.20g/kg, 0.60g/kg, 0.20g/kg ） could increase numbers of cone cell in CA3 region of hippocampus obviously （P〈0.01, P〈0.05, P〈0.05 ） . Conclusion HPE can improve the symptoms of the depression in the mouse model and protect injury of hippocampal neurons in the depression model mice.

关 键 词：贯叶金丝桃提取物 抑郁症 行为 海马神经元 

分 类 号：R971.43[医药卫生—药品]