芥菜型油菜和白菜型油菜杂种后代自交亲和性研究  

Study on Self-compatibility of Interspecific Hybrid Progeny between Brassica juncea and Brassica rapa

作　　者：牛妍[1] 赵志刚[1] 余青兰[1] 

机构地区：[1]青海大学农林科学院青海省高原作物种质资源创新与利用重点实验室培育基地/国家油菜改良中心青海分中心,青海西宁810016

出　　处：《河南农业科学》2013年第9期36-42,共7页Journal of Henan Agricultural Sciences

基　　金：国家重点基础研究发展计划("973"计划)前期研究项目(2011CB111508);"十二五"国家科技支撑计划项目(2011BAD35B04);青海省应用基础研究项目(2011-Z-701)

摘　　要：为获得自交亲和的白菜型油菜材料，利用芥菜型油菜和白菜型油菜种间杂交，将芥菜型油菜的自交亲和性状导入白菜型油菜，并揭示杂种后代自交亲和性状的渗入过程和状态。芥白杂种后代BC1F1群体的自交亲和指数介于0．05～0．88，群体自交不亲和，而BC1F2和BC2F1群体自交亲和指数最高达到14．33，自交亲和的植株分别占35．5％和22．2％。对BC1F2和BC2F1植株自交亲和性状分离进行统计和测验分析，表明白菜型油莱的自交亲和性状可能是单基因控制的显性遗传。BC1F3和BC2F2群体中自交亲和植株显著增多，分别占75．3％和87．59／6。对BC1F2代和BC1F3代植株花粉管萌发观察和自交亲和指数比较均表明，芥白杂种后代植株获得的自交亲和性状是可遗传的。In this study, the self-compatibility trait of B. juncea was transferred into B. rapa through interspecific hybridization between B. juncea and B. rapa and the introgression process and state of self-compatibility trait in hybrid progenies were revealed. The BC1F1 progeny was self-incompatible, of which self-compatible index varied from 0.05 to 0.88. However,in BC1Fz and BC2F1 progenies, the highest index reached 14. 33 and the percentage of self-compatible plants accounted for 35.5 % and 22.2 %, respectively. Analyzing self-compatible data of BC1 F2 and BC2F1 progenies satistically,the results showed that the self-compatibility of B. rapa was probably con- trolled by a couple of genes which managed dominant genetics. In BC1Fa and BC2Fz progenies, self-compatible plants significantly increased and accounted for 75.3% and 87.5%, respectively. By means of observing pollen tube germination and comparing self-compatibility indexes of plants in the BC1F2 progeny and BC1 F3 progeny, the results both show that the self-compatibility trait of hybrid progenies is inheritable.

关 键 词：芥菜型油菜 白菜型油菜 种间杂交 自交亲和 花粉管萌发 

分 类 号：S565.4[农业科学—作物学]