太行山羊SPRN基因的克隆与半定量RT-PCR分析  

Cloning and Semi-quantitative RT-PCR Analysis of SPRNgene in Taihang Goat

作　　者：张军杰[1] 周荣艳[1] 李祥龙[1] 陈辉[1] 张振红[1] 锡建中[1] 李兰会[1] 李秀岭 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]北京市大兴区动物疾病控制中心,北京102600

出　　处：《河南农业科学》2013年第9期116-119,共4页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31201775);河北省教育厅项目(Z2011152)

摘　　要：为研究太行山羊SPRN基因的结构及其在不同组织中的表达水平,参考绵羊和牛SPRN基因序列设计引物,应用PCR技术获得了太行山羊SPRN基因的核苷酸序列,同时对该基因进行生物信息学分析,并通过半定量RT-PCR进行组织表达谱分析。结果显示,克隆的太行山羊SPRN基因序列长度为4 237bp,含有441bp的完整开放阅读框,编码146个氨基酸,与绵羊和牛的对应基因序列同源性分别达到95%和93%。SPRN基因在山羊小脑和大脑中表达水平较高,在睾丸、肠系膜淋巴结和肺脏中表达量很低。To investigate the gene structure and tissue expression profile of SPRN gene in Taihang goat, the primers to amplify SPRN gene are designed based on sheep and cattle se- quences. The genomic sequence of caprine SPRN gene was acquired using PCR technology and tissue expre-ssion profile was analyzed using semi-RT-PCR technology. The bioinformatics analy- sis was done based on caprine SPRN gene sequence. The results showed that the length was 4 237 bp, including the complete open reading frame. The full-length of coding sequence was 441 bp,enco- ding 146 amino acid. The similarity between goat and sheep or cattle was 95% or 93% respective- ly. The expression profile showed that SPRN mRNA was highly expressed in cerebrum and cere- bellum, low levels in testis, mesentic lymph node,and lung and no mRNA was detected in other ti- ssues. The result of phylogenetic analysis was consistent with the taxonomy. The high expression level of SPRN gene in brain tissue is the important basis for the function research.

关 键 词：山羊 SPRN基因 克隆 RT—PCR 组织表达谱 

分 类 号：S828[农业科学—畜牧学]