药用植物通关藤中总黄酮提取工艺研究  

Study on the Extractive Technology of Flavonoids from Marsdenia tenacissima

作　　者：孟衡玲[1] 杨生超[2] 苏一兰[1] 文国松[2] 

机构地区：[1]红河学院云南省高校农作物优质高效栽培与安全控制重点实验室,云南红河661100 [2]云南农业大学云南省中药材规范化种植技术指导中心,云南昆明650201

出　　处：《河南农业科学》2013年第9期157-160,共4页Journal of Henan Agricultural Sciences

基　　金：国家支撑计划项目(2011BAI13B02-5);云南省重大产业项目[云发改高技(2007)1718号]

摘　　要：采用乙醇提取法提取通关藤中的总黄酮,利用正交试验研究提取温度、料液比、提取时间、乙醇体积分数对通关藤中总黄酮提取率的影响。结果表明,影响总黄酮提取率的因素依次为乙醇体积分数>料液比>提取时间>提取温度,最佳工艺为乙醇体积分数70%,料液比1∶20,提取时间3h,提取温度70℃,在最佳提取工艺条件下提取率为40.22mg/g。In this paper, ethanol extraction was used to obtain total Marsdenia tenacissima fla- vonoids,with rutin as control sample. The experiment used the orthogonal experimental design method to analyze the optimal extraction process of flavonoids from M. tenacissima. There were four factors affecting extraction efficiency, i. e. , the material ratio, extracting time, extraction tem- perature and mass fraction of ethanol. The results showed that the order of the four factors was： mass fraction of ethanol, material liquid ratio, extraction time, and extraction temperature. The op- timum technology was ： the extraction temperature of 70 ℃, material liquid ratio of 1 ： 20, mass fraction of ethanol 70% ,and extracting time 3 h. The extraction rate reached 40.22 mg/g in the best extraction conditions.

关 键 词：通关藤 总黄酮 提取工艺 

分 类 号：S567[农业科学—中草药栽培]