小鼠早期营养过剩诱导子代肥胖动物模型的建立  

Establishment of obesity model in offspring rats induced by early overfeeding

作　　者：纪金金[1] 董志[1] 

机构地区：[1]重庆医科大学药学院药理教研室,重庆400016

出　　处：《重庆医科大学学报》2013年第9期965-968,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金面上资助项目(编号:81173066);国家科技重大专项重大新药创制资助项目(编号:2010ZX09401-306-1-1);重庆市自然科学基金重点资助项目(编号:CSTC2009BA5086)

摘　　要：目的：观察小鼠早期营养过剩对子代肥胖的影响，为研究儿童肥胖机制提供实验基础。方法：通过减少哺乳期间幼鼠只数建立早期营养过剩动物模型即母代模型，实验组在子代出生第3天调整为3只，窝，对照组调整为10只／窝。母乳喂养21d后给予小鼠标准饲料喂养至第8周，与同龄正常雄性ICR小鼠交配，得早期营养过剩子代和正常小鼠子代，在小鼠出生第3天，均调整为10只／窝。每周测定子代体质量，母乳喂养21d后给予小鼠标准饲料喂养，并测定第23-29天进食量。在第30天处死小鼠．HE染色观察性腺周围白色脂肪组织形态。结果：第8周合笼时，早期营养过剩母代平均体质量为（40．75±0．89）g，正常母代平均体质量为（33．01±1．71）g（t=4．021，P=-0．007）。与正常小鼠子代相比，早期营养过剩小鼠的子代体质量增加[1（F=465．387，P=-0．000），进食量增加（t＝3．709，P=0．006），脂肪细胞体积增大（t＝4．487，P=0．001）。结论：小鼠早期营养过剩增加了子代肥胖的危险。Objective ：To investigate the effects of early overfeeding on the obesity of offspring rats and to provide references for experi-ment in child obesity. Methods：Early overfeeding female rat model was established on the 3rd d after birth by reducing the litter size to 3 pups per litter（small litter,SL）,which was in contrast to the normal litter size of 10 pups per litter（NL）. The pups were weaned onto regular chow after breast feeding for 21 d. At the 8th week, these rats were bred with normal male rats and offsprings of the early overfeeding and control rats weregenerated. Offsprings of both two groups were culled and raised as 10 pups per dam and weaned onto regular chow on the 21st d. Body weight and daily food intake were routinely recorded. Rats were killed on the 30th d and HE staining was performed to observe the morphology of epididymal fat. Results：Body weight of early overfeeding female rats was （40.75 ± 0.89） g while that of control female rats was （33.01± 1.71） g （t=4.021 ,P=0.007）. Compared with those of offspring of control rats,offspring of early overfeeding rats showed significant body weight gain （F=465.387, P=0.000） and hyperplasia （t=3.709, P=-0.006）. Adipocyte size in epididymal fat was larger in early overfeeding rats than in cortrol rats（t=4.487,P=0.001） based on HE staining observation. Conclusion：Maternal obesity can induce the risk of obesity in offspring.

关 键 词：母代 早期营养过剩 子代 肥胖 

分 类 号：R589.2[医药卫生—内分泌]