精液常规参数对卵胞浆内单精子显微注射治疗结局的影响  

Effects of sperm parameters on outcomes of intracytoplasmic sperm injection

作　　者：高洋[1] 黄国宁[2] 王亚平[1] 

机构地区：[1]重庆医科大学干细胞与组织工程研究室、组织胚胎学教研室,重庆400016 [2]重庆市妇幼保健院遗传与生殖研究所,重庆400013

出　　处：《重庆医科大学学报》2013年第9期1081-1084,共4页Journal of Chongqing Medical University

摘　　要：目的:分析精液常规参数对卵细胞浆内单精子显微注射(intracytoplasmic sperm injection,ICSI)治疗结局的影响。方法:对290例ICSI治疗周期进行回顾性分析,根据精子参数分为:A(精液常规正常和轻度异常)、B(严重少精)、C(严重弱精)、D(严重畸精)和E(严重混合精子异常)5组。比较各组的受精率、卵裂率、可移植胚胎率、优质胚胎率、着床率和妊娠率是否存在差异。结果:5组的受精率、卵裂率、可移植胚胎率和优质胚胎率比较,差异无统计学意义(χ2=1.27,P=0.87;χ2=1.34,P=0.86;χ2=0.56,P=0.97;χ2=1.39,P=0.85)。D组和E组的生化妊娠率、着床率和临床妊娠率均低于A组(χ2=12.1,P=0.00;χ2=10.15,P=0.00;χ2=10.78,P=0.00;χ2=9.90,P=0.00;χ2=8.13,P=0.00;χ2=11.78,P=0.00)、B组(χ2=6.04,P=0.01;χ2=7.79,P=0.01;χ2=7.21,P=0.01;χ2=4.29,P=0.04;χ2=5.63,P=0.02;χ2=7.21,P=0.01)和C组(χ2=7.01,P=0.01;χ2=6.47,P=0.01;χ2=6.95,P=0.01;χ2=5.14,P=0.02;χ2=4.35,P=0.04;χ2=7.01,P=0.01)。结论:不同参数的精子不影响ICSI治疗的受精结局和胚胎质量,严重形态异常的精子行ICSI治疗后着床率和妊娠率降低。Objective：To evaluate the effects of sperm with different parameters on the outcomes of intracytoplasmic sperm injection （ICSI）. Methods ：Totally 290 ICSI cycles were analyzed retrospectively. They were divided into 5 groups according to sperm parame ters：group A （normal sperm forms or mild oligoastheno-teratozoospermia）, group B （severe oligozoospermia）, group C （severe astheno zoospermia）, group D （severe teratozoospermia）, group E （ severe oligoastheno-teratozoospermia）. Fertilization rate, cleavage rate, transferable embryos rate, good quality embryos rate, implantation and pregnancy rates were compared between the groups. Results： Fertilization rate, cleavage rate,transferable embryos rate and good quality embryos rate were not significantly different among the 5 groups （x2= 1.27,x2=-0.87; X2= 1.34, P=-0.86; X2=0.56, P=0.97; 2,2= 1.39,P=-0.85）. The chemical pregnancy rate, embryo implantation rate and clinical pregnancy rate were lower in group D and E than in group A （X2=12.1, P=0.00;）（2=10.15,P=0.00;x2= 10.78,P=0.00; x2= 9.90, P=0.00 ; X2=8.13, P=0.00; x2=l 1.78, P=0.00）, B （X2=6.04, P=0.01 ; ）（2=7.79, P=0.01 ; X2=7.21, P=0.01 ; ）（2=4.29, P=0.04; ）（2=5.63, P=0.02 ; X2=7.21 ,P=0.01 ）and C （X2=7.01 ,P=0.01 ; X2=6.47, P=0.01 ; X2=6.95 ,P=0.01 ; X2=5.14,P=0.02 ; X2=4.35 ,P=0.04 ; X2=7.01 ,P= 0.01 ）. Conclusions：Sperms of different parameters used in ICSI treatment can not affect the fertilization rate. Severe teratozoospermia can reduce the implantation and pregnancy rates after ICSI treatment.

关 键 词：卵胞浆内单精子注射 精子参数 临床结局 辅助生殖 

分 类 号：R711.6[医药卫生—妇产科学]