转录因子Klf4在人牙髓组织和牙髓成纤维细胞中的表达研究  

Expression of Klf4 in human dental pulp tissue and fibroblasts

作　　者：汪涵 陈卓[2] 

机构地区：[1]杭州口腔医院特需科,杭州310000 [2]浙江大学医学院附属口腔医院牙体牙髓科,杭州310000

出　　处：《口腔医学》2013年第9期577-580,共4页Stomatology

基　　金：国家自然科学基金资助项目(81100727);浙江省教育厅基金资助项目(Y200909390);国家临床重点专科建设项目

摘　　要：目的研究转录因子Klf4在人牙髓组织和体外培养的牙髓成纤维细胞中的表达特点。方法采用免疫荧光技术检测Klf4和增殖标记物ki67在人牙髓组织及体外培养的牙髓成纤维细胞中的表达情况;利用逆转录聚合酶链反应(RT-PCR)检测Klf4在人牙髓细胞和矿化诱导14 d的表达情况。结果人牙髓中,Klf4主要表达在成牙本质样细胞和血管内皮细胞中;在体外培养的牙髓成纤维细胞中,与诱导前相比,Klf4在矿化诱导14 d时的表达明显增强。结论 Klf4在成牙本质细胞中呈特异性表达,可能与成牙本质细胞分化相关。Objective To study the expression pattern of Klf4 in human dental pulp and fibroblasts in vitro. Methods [mmunoflu- orescence was used to obtain the expression of Klf4 and Ki67 in human dental pulp and fibroblasts in vitro. Reverse transcriptase-poly- merase chain reaction was used to detect the mRNA expression of Klf4 in human dental pulp cells and inducing mineralization on day 14. Results In human dental pulp,the expression of Klf4 was positive in odontoblasts and vascular endothelial cells; in fibroblasts in vitro, the expression of Klf4 was up-regulated in inducing mineralization on day 14. Conclusions Klf4 was specifically expressed in odontoblasts, and it may be correlated with the differentiation of odontoblasts.

关 键 词：KLF4 牙髓 表达 免疫荧光 

分 类 号：R349.64[医药卫生—基础医学]