Gaussia分泌型萤光素酶的研究进展及其应用  

Research Progress and Application of the Secreted Gaussia Luciferase

作　　者：张秀娟[1,2] 黄海[2] 谢佩雯[2] 王学军[2] 王升启[2] 

机构地区：[1]天津中医药大学,天津300193 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《生物技术通讯》2013年第5期722-726,共5页Letters in Biotechnology

基　　金：国家自然科学基金(81202562);国家重大新药创制专项(2012ZX09103301-044)

摘　　要：Gaussia分泌型萤光素酶是近年发现的一种来源于海洋桡脚类动物Gaussia princeps的新型分泌型萤光素酶,是目前已知的可自然分泌的最小萤光素酶,因其分子小、灵敏度高、半衰期短和可高效分泌,而成为一种理想的报告基因,广泛应用于体内外研究。我们就Gaussia分泌型萤光素酶的发光原理、荧光特性及其应用等进行简要综述。Gaussia luciferase is a novel secreted luciferase derived from the marine copepod Gaussia princeps, known as the smallest naturally secreted luciferase. Because of its small molecular weight, high sensitivity, short half-life, and efficient secretion, it is applied as an ideal reporter gene which has been widely used in the in vi-tro and in vivo studies. The luminescence principle, characteristics, and application of Gaussia luciferase were re-viewed in this article.

关 键 词：Gaussia萤光素酶 报告基因 荧光特性 

分 类 号：Q52[生物学—生物化学]