纳米银对人肝癌细胞及正常肝细胞增殖与凋亡的影响  

Effects of proliferation and apoptosis induced by silver nanoparticles in HepG2 cells and L02 cells

作　　者：黄艳梅[1] 巩凡[1] 张婷[1] 胡媛媛[1] 薛玉英[1] 唐萌[1] 

机构地区：[1]东南大学公共卫生学院环境医学工程教育部重点实验室江苏省生物材料与器件重点实验室,江苏南京210009

出　　处：《环境与健康杂志》2013年第9期770-772,F0003,共4页Journal of Environment and Health

基　　金：国家重大科学研究计划项目(2011CB933404);国家自然科学基金(30972504;81172697);江苏省自然科学基金(BK2011606);东南大学科技基金(KJ2010440)

摘　　要：目的探讨纳米银对人肝癌（HepG2）细胞株及人正常肝（L02）细胞株体外增殖与凋亡的影响。方法向HepG2细胞和L02细胞培养液中分别加入0（对照）-640μg／ml纳米银溶液染毒24、48h。采用MTT比色法检测细胞活性；采用流式细胞术检测细胞早期凋亡率。结果与对照组比较，染毒24、48h后纳米银暴露组HepG2细胞和L02细胞的存活率均较低，而凋亡率均较高；且随着纳米银暴露剂量的升高，HepG2细胞和L02细胞的存活率均呈下降趋势，而凋亡率均呈上升趋势。与染毒48h组比较，染毒24h后纳米银暴露组HepG2细胞和L02细胞的存活率均较高，而凋亡率均较低。与相同染毒时间HepG2细胞比较，染毒24、48h后纳米银暴露组L02细胞的存活率均较高，而凋亡率均较低。结论纳米银对L02细胞有一定的细胞毒性，能抑制细胞增殖，诱导细胞凋亡；但其对HepG2细胞的毒性更强。Objective To explore the influence of silver nanoparticles on the proliferation and apoptosis of human hepatoma cell line （HepG2） cells and human normal liver cell line （L02） cells in vitro. Methods The HepG2 cells and the L02 cells were used. The two cell lines were treated with silver nanoparticles at the level of 0-640 μg/ml for 24 h and 48 h in vitro. The proliferation of cells was determined by MTT assay. The apoptosis rate of cells was indentified by flow eytometry assay. Results After 24 h or 48 h of treatment, the survival rates of HepG2 cells and L02 cells were lower than the control groups, while the apoptosis rates of the two cell lines were higher than the control groups. With the increase of exposure concentration, the survival rates showed a decreased tendency and the apoptosis rates showed an increasing tendency in both HepG2 cells and L02 cells. The survival rates of HepG2 cells and L02 cells were higher in 24 h-treatment groups than in 48 h-treatment groups, while the apoptosis rates of the two cell lines were lower in 24 h-treatment groups than in 48 h-treatment groups. The survival rates of the L02 cells were significantly higher compared with the HepG2 cells treated with the same exposure time, while the apoptosis rates were lower. Conclusion Silver nanoparticles may present eytotoxicity to HepG2 and L02 cell lines by inhibiting the proliferation and inducing the apoptosis of cells. The toxic effect induced by silver nanoparticles on HepG2 cells is stronger than on L02 cells.

关 键 词：纳米银 HEPG2细胞 L02细胞 增殖抑制 细胞凋亡 

分 类 号：R994.6[医药卫生—毒理学]