山茱萸环烯醚萜总苷对晚期糖基化终末产物诱导的肾系膜炎症反应的调节  

Regulation of iridoid glucosides from Corni officinalis Fructus on AGEs-induced inflammatory response in renal mesangial cells

作　　者：郭洁[1] 张晓双[1] 刘继平[1] 

机构地区：[1]陕西中医学院药理教研室,陕西咸阳712046

出　　处：《中成药》2013年第10期2067-2072,共6页Chinese Traditional Patent Medicine

摘　　要：目的探讨山茱萸环烯醚萜总苷对晚期糖基化终末产物(AGEs)诱导的大鼠肾系膜HBZY-1细胞炎症反应的影响以及可能的作用机制。方法采用巨噬细胞和肾系膜细胞共培养体系,评估巨噬细胞的迁移能力;采用ELISA试剂盒评估炎症因子的分泌水平;采用Western blot分析法评估炎症相关蛋白的表达。结果与AGEs诱导的模型组相比,山茱萸环烯醚萜总苷显著抑制AGEs诱导的巨噬细胞迁移能力,不同剂量山茱萸环烯醚萜总苷(1.0、0.5、0.25mg/mL),对巨噬细胞迁移抑制率分别为42.09%±7.25%、26.44%±10.23%、20.84%±6.84%;山茱萸环烯醚萜总苷显著抑制AGEs诱导的炎症因子IL-6,IL-10,MCP-1和TNF-α的分泌水平;Western blot分析显示山茱萸环烯醚萜总苷及AGEs受体特异性抗体显著下调AGEs诱导的ICAM-1和TGF-β1的蛋白表达。结论山茱萸环烯醚萜总苷可剂量依赖性地减轻AGEs诱导的大鼠肾系膜细胞HBZY-1炎症反应,对糖尿病肾病的发生发展具有一定的保护作用。AIM To investigate the regulation of iridoid glycoside from Corni officinalis Fructus on advanced glycation end-products （AGEs） -induced inflammatory response in rat renal mesangial HBZY-1 cell. METHODS The macrophage and renal mesangial cells co-culture system was established to assess the migration of macropha- ges to mesangial cells. ELISA kits were used to evaluate the secretion level of inflammatory cytokines. Western blot analysis was developed to assess the inflammation-related protein expression. RESULTS Compared with AGEs- induced model group, iridoid glycoside from Corni officinalis Fructus significantly inhibited AGEs-induced the mi- gration ability of macrophage to mesangial cells. The migration inhibitory rates of different doses of iridoid glycoside from Corni officinalis Fructus （ 1.0 , 0. 5 and 0. 25 mg/mL） were 42.09% ± 7.25% , 26. 44% ± 10. 23% and 20. 84% ± 6.84% , respectively. The pretreatment with iridoid glycoside from Corni officinalis Fructus significantly inhibited the levels of inflammatory cytokines induced by AGEs, including IL-6, IL-10, MCP-1 and TNF-α secre- tion levels. Furthermore, western blot analysis showed that iridoid glycoside from Corni officinalis Fructus and re- ceptor for AGEs antibody （ RAGE-Ab, 5 μg/mL） significantly attenuated AGEs-induced ICAM-1 and TGF-β1 pro- tein expression. CONCLUSION Iridoid glycoside from Corni officinalis Fructus could attenuate AGEs-induced inflammatory response in rat mesangial cells HBZY-1 in a dose-dependent manner via blocking AGEs-RAGE path- way, and has a protective effect on the development of diabetic nephropathy.

关 键 词：山茱萸环烯醚萜总苷 炎症反应 肾系膜细胞 晚期糖基化终末产物 大鼠 

分 类 号：R285.5[医药卫生—中药学]