LC-MS/MS法同时测定维C银翘片中6种成分  

Determination of six constituents in Vitamin C Yinqiao Tablets by LC-MS/MS

作　　者：杨晓 杨钊 李新荣 

机构地区：[1]青岛市药品检验所,山东青岛266071

出　　处：《中成药》2013年第10期2153-2156,共4页Chinese Traditional Patent Medicine

摘　　要：目的 建立同时测定维C银翘片中对乙酰氨基酚、马来酸氯苯那敏、绿原酸、牛蒡苷、连翘苷、甘草苷的高效液相-串联四级杆质谱方法.方法 采用Waters系统,Kromasil 100-5 C18（150mm×2.1 mm,3μm）色谱柱分离,多反应监测模式测定,乙腈-0.1％甲酸溶液梯度洗脱,体积流量0.2 mL/min；柱温35℃；进样量10 μL.ESI＋离子化模式.结果 6种成分在28 min内即达到基线分离,在测定的浓度范围内线性关系良好,r分别在0.992 6～0.999 6之间,各成分的平均回收率在82.1％ ～96.1％之间.结论 该方法准确、简便、灵敏,可作为维C银翘片的质量控制方法.AIM To establish a method for simultaneously determining six constituents （acetaminophen, ehlorphenamine maleate, ehlorogenie acid, arctiin, forsythin and liquiritin） in Vitamin C Yinqiao Tablets by LC triple-quadrupole tandem mass spectrometer. METHODS The multi-reactions monitoring （MRM） technology was used. The separation was performed on Kromasil 100-5 C18 （150 mm ×2. 1 mm, 3 μm） column with aeetonitrile- 0. 1% formic acid with flow rate of 0.2 mL/min in gradient elution manner at temperature of 35 ℃. Ion mode was ESI ＋. RESULTS Six constituents could be well separated at baseline in 28 min. The calibration curve was linear in the measurement concentration range （r = 0. 992 6 -0. 999 6 ）. The average recovery was from 82. 1% to 96. 1% with RSD less than 3% . CONCLUSION The method is rapid, sensitive and accurate with good repro- ducibility, and is available for quality control of Vitamin C Yinqiao Tablets.

关 键 词：液相色谱质谱联用 维C银翘片 对乙酰氨基酚 马来酸氯苯那敏 绿原酸 牛蒡苷 连翘苷 甘草苷 

分 类 号：R927.2[医药卫生—药学]