[Cu(Glu·Arg)]SO_4·5H_2O与牛血清白蛋白(BSA)作用的荧光光谱分析  

Flouorescence spectrometric study of interaction between complex [Cu(Glu·Arg)]SO_4·5H_2O and bovine serum albumin

作　　者：殷林波[1] 滕文锋[1] 

机构地区：[1]大连医科大学检验医学院,辽宁大连116044

出　　处：《化学试剂》2013年第10期894-896,911,共4页Chemical Reagents

摘　　要：以Cu(Ⅱ)作为中心离子,谷氨酸(Glu)和精氨酸(Arg)作为配体合成了铜离子混配合物[Cu(Glu·Arg)]SO4·5H2O;利用荧光光谱技术对该混配合物与牛血清白蛋白的(BSA)的相互作用进行了研究分析。结果表明:混配合物与BSA相互作用,可以使BSA产生内源性荧光猝灭,猝灭机理为静态猝灭,表观猝灭常数为K q=1.2×1012L·mol-1·s-1,结合常数为K=9.7×103L/mol,结合位点数为n=0.966。The binding interaction between the mixed comple- xes （ [ Cu （ Glu- Arg） ] SO4·5 H2O ） and bovine serum albumin （BSA） was investigated by fluorescence spectroscopy. The mixed complexes was designed and synthesized with Cu（ II ）, glutamate（Glu） and arginine （Arg）. The mixed complexes have powerful ability to quench the BSA fluorescence via a static quenching mechanism and quenching rate constant is 1.2 ×10^12L·mol^-1·s^-1 and binding constant is 9.7 ×10^3L/mol and the number of binding site is 0. 966.

关 键 词：铜(Ⅱ)混配合物 牛血清白蛋白 荧光猝灭 

分 类 号：O657.34[理学—分析化学]